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View additional product information for NanoDrop™ Reagents - FAQs (CHEM-PV-8, CHEM-CF-8, CHEM-PR1-KIT, CHEM-PV-1, CHEM-CF-1)
10 product FAQs found
Our NanoDrop CF-1 Calibration Fluid should be used with the Calibration Check module of the instrument software. CF-1 is prepared from the NIST potassium dichromate standard SRM935 in acidified reagent grade water (Certificate of Analysis available from NanoDrop products).
We recommend confirming that your NanoDrop Lite Spectrophotometer is working within specifications every 6 months. The NanoDrop CF-1 Calibration Fluid is required to run the calibration check procedure. CF-1 is available from Thermo Fisher website (Cat. No. CHEM-CF-1)
The accuracy of your NanoDrop Lite Spectrophotometer can be checked by using the NanoDrop CF-1 Calibration Fluid to perform a calibration check. CF-1 is prepared from the NIST potassium dichromate standard SRM935 in acidified reagent grade water.
Please review the following steps to take when/if your NanoDrop 8000 Spectrophotometer fails a calibration check:
- Clean the pedestal with the NanoDrop PR-1 Reconditioning Kit
- Repeat calibration check using a FRESH vial of NanoDrop CF-1 Calibration Fluid and FRESH blanking solution
- If instrument continues to be out of specification, Contact Technical Support
You can easily check the calibration of your NanoDrop 8000 Spectrophotometer by using the NanoDrop CF-8 Calibration Check Kit, which contains two CF-1 Ampules and two 8-well PCR tube strips. The CF-8 kit is available from the Thermo Fisher website (Cat. No. CHEM-CF-8).
If re-calibration is ever needed, it is performed by a trained service technician. Due to the precise nature of the NanoDrop instrument design, these spectrophotometers rarely require re-calibration.
Please review the following steps to take when/if your NanoDrop 2000/2000c fails a calibration check:
- Clean the pedestal with the NanoDrop PR-1 Reconditioning Kit
- Repeat calibration check using a FRESH vial of NanoDrop CF-1 Calibration Fluid and FRESH blanking solution
More detailed instructions can be found on the Calibration Check Procedure document: https://assets.thermofisher.com/TFS-Assets/CAD/Product-Bulletins/NanoDrop-2000-2000c-1000-Calibration-Check-procedure.pdf
If your NanoDrop 2000/2000c continues to be out of specification, Contact Technical Support.
We recommend you check the calibration of your NanoDrop 2000/2000c every 6 months using the NanoDrop CF-1 Calibration Fluid.
You can easily check the calibration of your NanoDrop 2000/2000c Spectrophotometer by using NanoDrop CF-1 Calibration Fluid. CF-1 is specifically designed for NanoDrop spectrophotometers and is available from the Thermo Fisher website (Cat. No. CHEM-CF-1).
If re-calibration is ever needed, it is performed by a trained service technician. Due to the precise nature of the NanoDrop instrument design, these spectrophotometers seldom require re-calibration.
NanoDrop CF-1 Calibration Fluid is the only photometric standard available to assess the accuracy of the NanoDrop spectrophotometer pedestal. If the control sample shows unexpected results the user should confirm the calibration of the instrument with the CF-1 fluid.
Some control examples include:
- Nucleic Acids: Fermentas GeneRuler Express DNA Ladder 100-5000 bp (Cat. No. SM1551)
- Protein: Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209)
The accuracy of NanoDrop spectrophotometers can be assessed by using NanoDrop CF-1 Calibration Fluid in conjunction with the calibration check application in the NanoDrop software.