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Invitrogen
JMJD1A Polyclonal Antibody
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FIGURE: 1 / 5
JMJD1A Antibody (PA5-88279) in ICC/IF
Immunofluorescence analysis of JMJD1A was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KDM3A Polyclonal Antibody (Product # PA5-88279) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-88279
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 975-1283 of human KDM3A (NP_0609032)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.88 mg/mL
Amount
88 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: 293T; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: PVMVSGVHHK LNSELWKPES FRKEFGEQEV DLVNCRTNEI ITGATVGDFW DGFEDVPNRL KNEKEPMVLK LKDWPPGEDF RDMMPSRFDD LMANIPLPEY TRRDGKLNLA SRLPNYFVRP DLGPKMYNAY GLITPEDRKY GTTNLHLDVS DAANVMVYVG IPKGQCEQEE EVLKTIQDGD SDELTIKRFI EGKEKPGALW HIYAAKDTEK IREFLKKVSE EQGQENPADH DPIHDQSWYL DRSLRKRLHQ EYGVQGWAIV QFLGDVVFIP AGAPHQVHNL YSCIKVAEDF VSPEHVKHCF WLTQEFRYL
Target Information
JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686A24246; DKFZp686P07111
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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