Vybrant™ 细胞代谢检测试剂盒，含 C12-刃天青
Vybrant&trade; 细胞代谢检测试剂盒，含 C<sub>12</sub>-刃天青
引用和文献 (7)
Invitrogen™

Vybrant™ 细胞代谢检测试剂盒，含 C12-刃天青

在 Vybrant™ 细胞代谢测定试剂盒中，无荧光的 C12-刃天青被活细胞还原为具有荧光的 C12-试卤灵；产生的信号与存在的细胞数量成正比。C12-刃天青比 alamarBlue™ 染料具有更好的细胞保留能力，并且可以与细胞活力指示剂及其他生物标记物一起进行多通路检测了解更多信息
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货号数量
V231101 kit
货号 V23110
价格（CNY)
5,445.00
1 kit
添加至购物车
数量:
1 kit
价格（CNY)
5,445.00
1 kit
添加至购物车
在 Vybrant™ 细胞代谢测定试剂盒中，无荧光的 C12-刃天青被活细胞还原为具有荧光的 C12-试卤灵；产生的信号与存在的细胞数量成正比。C12-刃天青比 alamarBlue™ 染料具有更好的细胞保留能力，并且可以与细胞活力指示剂及其他生物标记物一起进行多通路检测
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型试卤灵
产品规格管、96 孔板
数量1 kit
运输条件室温
适用于（应用）细胞代谢测定试剂盒
适用于（设备）流式细胞仪, 微孔板读数仪
产品线Vybrant
产品类型染料
Unit Size1 kit
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

alamarBlue试剂和PrestoBlue试剂与刃天青和C12-刃天青有什么不同？

alamarBlue试剂和PrestoBlue试剂含有的刃天青包含在一种专有的稳定配方中，能够提供了一种便捷的“混合、接种和读数”实验方案。PrestoBlue试剂是alamarBlue试剂的改进配方允许更快速的染色（典型的10分钟相比于1-4小时获得相似的信号和灵敏度）。C12-刃天青是刃天青的衍生物，其拥有良好的细胞滞留性，因此适用于伴有活性指示剂和其他生物标记的流式细胞分析仪多重检测分析。

How do alamarBlue reagent and PrestoBlue reagent differ from resazurin and C12-resazurin?

alamarBlue reagent and PrestoBlue reagent contain resazurin in a proprietary stabilizing formulation that allows for a convenient “mix, incubate, and read” protocol. PrestoBlue reagent is an improvement in the formulation of alamarBlue reagent that allows for much faster staining (typically 10 minutes vs. 1-4 hours to obtain a similar signal and sensitivity). C12-resazurin is a derivative of resazurin that has better cellular retention and thus allows for analysis on a flow cytometer and multiplexing with viability indicators and other biomarkers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the absorbance/emission of the reaction product from the Vybrant Cell Metabolic Assay Kit?

The absorbance/emission is 563/587 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (7)

引用和文献
Abstract
Membrane disruption by antimicrobial fatty acids releases low-molecular-weight proteins from Staphylococcus aureus.
Authors:Parsons JB, Yao J, Frank MW, Jackson P, Rock CO,
Journal:J Bacteriol
PubMed ID:22843840
The skin represents an important barrier for pathogens and is known to produce fatty acids that are toxic toward gram-positive bacteria. A screen of fatty acids as growth inhibitors of Staphylococcus aureus revealed structure-specific antibacterial activity. Fatty acids like oleate (18:1?9) were nontoxic, whereas palmitoleate (16:1?9) was a potent growth ... More
Perturbational profiling of nanomaterial biologic activity.
Authors:Shaw SY, Westly EC, Pittet MJ, Subramanian A, Schreiber SL, Weissleder R,
Journal:Proc Natl Acad Sci U S A
PubMed ID:18492802
'Our understanding of the biologic effects (including toxicity) of nanomaterials is incomplete. In vivo animal studies remain the gold standard; however, widespread testing remains impractical, and the development of in vitro assays that correlate with in vivo activity has proven challenging. Here, we demonstrate the feasibility of analyzing in vitro ... More
Novel designs of multivalent anti-CD20 humanized antibodies as improved lymphoma therapeutics.
Authors:Rossi EA, Goldenberg DM, Cardillo TM, Stein R, Wang Y, Chang CH,
Journal:Cancer Res
PubMed ID:18922911
'Multivalent antibodies, either monospecific or bispecific, may improve the efficacy of current therapeutic interventions involving a single monoclonal antibody (mAb). We have applied the Dock-and-Lock (DNL) method, a new platform technology for the site-specific and covalent assembly of modular components into stably tethered complexes of defined composition, to prepare a ... More
Preclinical studies on targeted delivery of multiple IFNa2b to HLA-DR in diverse hematologic cancers.
Authors:Rossi EA, Rossi DL, Cardillo TM, Stein R, Goldenberg DM, Chang CH,
Journal:Blood
PubMed ID:21680794
The short circulating half-life and side effects of IFNa affect its dosing schedule and efficacy. Fusion of IFNa to a tumor-targeting mAb (mAb-IFNa) can enhance potency because of increased tumor localization and improved pharmacokinetics. We used the Dock-and-Lock method to generate C2-2b-2b, a mAb-IFNa comprising tetrameric IFNa2b site-specifically linked to ... More
Hypothermia reduces secretion of vascular endothelial growth factor by cultured retinal pigment epithelial cells.
Authors:Coassin M, Duncan KG, Bailey KR, Singh A, Schwartz DM,
Journal:Br J Ophthalmol
PubMed ID:20805126
Visual loss in age-related macular degeneration usually develops secondary to choroidal neovascularisation. Vascular endothelial growth factor (VEGF) is a critical regulator of retinal angiogenesis and vascular permeability, especially in hypoxic conditions. We hypothesise that hypothermia may reduce the retinal pigment epithelium (RPE) metabolism and, consequently, the levels of VEGF secretion ... More
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