Membrane disruption by antimicrobial fatty acids releases low-molecular-weight proteins from Staphylococcus aureus.
AuthorsParsons JB, Yao J, Frank MW, Jackson P, Rock CO,
JournalJ Bacteriol
PubMed ID22843840
The skin represents an important barrier for pathogens and is known to produce fatty acids that are toxic toward gram-positive bacteria. A screen of fatty acids as growth inhibitors of Staphylococcus aureus revealed structure-specific antibacterial activity. Fatty acids like oleate (18:1?9) were nontoxic, whereas palmitoleate (16:1?9) was a potent growth ... More
Perturbational profiling of nanomaterial biologic activity.
'Our understanding of the biologic effects (including toxicity) of nanomaterials is incomplete. In vivo animal studies remain the gold standard; however, widespread testing remains impractical, and the development of in vitro assays that correlate with in vivo activity has proven challenging. Here, we demonstrate the feasibility of analyzing in vitro ... More
Novel designs of multivalent anti-CD20 humanized antibodies as improved lymphoma therapeutics.
AuthorsRossi EA, Goldenberg DM, Cardillo TM, Stein R, Wang Y, Chang CH,
JournalCancer Res
PubMed ID18922911
'Multivalent antibodies, either monospecific or bispecific, may improve the efficacy of current therapeutic interventions involving a single monoclonal antibody (mAb). We have applied the Dock-and-Lock (DNL) method, a new platform technology for the site-specific and covalent assembly of modular components into stably tethered complexes of defined composition, to prepare a ... More
Preclinical studies on targeted delivery of multiple IFNa2b to HLA-DR in diverse hematologic cancers.
The short circulating half-life and side effects of IFNa affect its dosing schedule and efficacy. Fusion of IFNa to a tumor-targeting mAb (mAb-IFNa) can enhance potency because of increased tumor localization and improved pharmacokinetics. We used the Dock-and-Lock method to generate C2-2b-2b, a mAb-IFNa comprising tetrameric IFNa2b site-specifically linked to ... More
Hypothermia reduces secretion of vascular endothelial growth factor by cultured retinal pigment epithelial cells.
AuthorsCoassin M, Duncan KG, Bailey KR, Singh A, Schwartz DM,
JournalBr J Ophthalmol
PubMed ID20805126
Visual loss in age-related macular degeneration usually develops secondary to choroidal neovascularisation. Vascular endothelial growth factor (VEGF) is a critical regulator of retinal angiogenesis and vascular permeability, especially in hypoxic conditions. We hypothesise that hypothermia may reduce the retinal pigment epithelium (RPE) metabolism and, consequently, the levels of VEGF secretion ... More
Mechanisms of palatal epithelial seam disintegration by transforming growth factor (TGF) beta3.
AuthorsAhmed S, Liu CC, Nawshad A,
JournalDev Biol
PubMed ID17698055
TGFbeta3 signaling initiates and completes sequential phases of cellular differentiation that is required for complete disintegration of the palatal medial edge seam, that progresses between 14 and 17 embryonic days in the murine system, which is necessary in establishing confluence of the palatal stroma. Understanding the cellular mechanism of palatal ... More
Genetic algorithm as an optimization tool for the development of sponge cell culture media.