TheraPure 2ʹ-OMe-U Phosphoramidite

2'-OMe-U CEP is a phosphoramidite containing 5' DMT and the nucleoside uridine (U) with 2'-O-methyl protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, 2'-O-methyl (OMe) oligo modifications display nuclease resistance, fewer off-target effects, and slightly increased hybridization affinities. These phosphoramidites are commonly used for oligos designed for antisense, siRNA, aptamer, or gRNA-based research. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in an amber bottle.

Available with a Serum Vial, 20-400 Finish, or 28-400 Finish neck style.

• Serum vial neck style is compatible with most Applied Biosystems synthesizers and AKTA synthesizers.
• 20-400 finish neck style is compatible with most Expedite synthesizers.
• 28-400 finish neck style is compatible with most MerMade synthesizers.

Features:

• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-uridine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C₄₀H₅₆N₇O₉P
• Base—U
• Formula weight—760.8 g/mol
• CAS number—110764-79-9
• HPLC purity—≥99%; M-11 Impurity: ≤0.01%; Single critical impurity ≤0.3%
• ³¹P NMR purity—>99%; Sum of non-primary peaks at 140-152 ppm ≤0.5%