Custom Plus TaqMan™ RNA Assay, FAM
Custom Plus TaqMan™ RNA Assay, FAM
Applied Biosystems™

Custom Plus TaqMan™ RNA Assay, FAM

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Applied Biosystems Custom Plus TaqMan RNA assays, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR.Read more
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Catalog NumberQuantity
4441114S (360 reactions), made to order
4441118L (2900 reactions), made to order
4441117M (750 reactions), made to order
Catalog number 4441114
Price (CNY)
4,208.00
Each
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Quantity:
S (360 reactions), made to order

Applied Biosystems Custom Plus TaqMan RNA assays, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR. Custom Plus TaqMan RNA assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Custom content—easily design an assay for over 20 species
• Designed using the full bioinformatic power of the TaqMan Assay design pipeline, which uses proprietary algorithms that have generated millions of assay designs and is regarded as the benchmark in the industry for qPCR design

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Recommended master mix: TaqMan Fast Advanced Master Mix

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Green FeaturesSustainable packaging
No. of Reactions360 reactions, made to order
Product LineTaqMan
Product TypeCustom TaqMan Gene Expression
QuantityS (360 reactions), made to order
Shipping ConditionRoom Temperature
Concentration20X
Unit SizeEach
Contents & Storage
1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

Frequently asked questions (FAQs)

When I click on a TaqMan Gene Expression assay ID, the results page shows that it detects several RefSeq and/or GenBank transcripts. Does this mean the assay detects different genes?

No, the assay is not detecting different genes. Genes often have multiple valid accession numbers, even for the exact same sequence. Even though there are different ID numbers listed, they should all represent the same gene.

What is the date printed on the label of the TaqMan Gene Expression Assays?

The label on the TaqMan Gene Expression Assays lists the expiration date, which under proper storage conditions is 5 yrs. The assay itself is guaranteed for 1 yr upon shipmentt.

When I search for a particular TaqMan Gene Expression Assay, there are multiple assay IDs. What are the differences between them?

We often design multiple assays for the same target. The difference between the assay IDs is most often that the assay is designed across a different exon-exon boundary of a particular transcript sequence. You can confirm the exon-exon boundary by clicking on the individual Assay ID and then look at the Interrogated Sequence Table.

Another difference between assays for the same gene is that they detect different mRNA species or splice variants for the same gene. Ensure that the assay you select will detect your transcripts of interest.

Can I get a custom TaqMan Gene Expression Assay synthesized with different reporter dyes?

No, the Custom TaqMan Gene Expression Assays are only synthesized with FAM dye as the reporter dye at the 5' end and MGB-NFQ as the quencher at the 3' end. Dye selection is only possible with general custom oligo synthesis.

In order to take advantage of our superior in-house assay design algorithm, we recommend that you order a small scale of the standard Custom TaqMan Gene Expression Assay with the FAM label first and validate the assay in a single-plex reaction. You can then submit the sequences of the primers and probes for custom oligo synthesis where you will have the flexibility of choosing your reporter dye.

Does a TaqMan Gene Expression Assay for one species cross-react with another species if there is high degree of sequence homology?

For the 23 species that we cover, you can use the species filter to narrow your results. This is located in the right-hand column of your assay search results. Look for “Exclude Assays that Detect”. Only species that will cross-react are listed (of the 23 species we screen against). Check the species box to remove assays that would detect in that particular species.

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