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Ion Torrent™
Oncomine™ Tumor Mutation Load Assay, Chef-ready library preparation
The Oncomine Tumor Mutation Load Assay is a targeted next-generation sequencing (NGS) assay that provides an assessment of tumor mutationRead more
| Catalog Number | Quantity |
|---|---|
| A37910 | 32 Samples |
Catalog number A37910
Price (CNY)
-
Quantity:
32 Samples
The Oncomine Tumor Mutation Load Assay is a targeted next-generation sequencing (NGS) assay that provides an assessment of tumor mutation load and mutation signatures in a simple workflow. The assay provides reagents for library construction from two pools of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin-embedded (FFPE) tumor samples without the need for a matched normal sample, using 10 ng of DNA per primer pool. This assay is designed to facilitate successful selection and identification of samples most likely to derive responses in cancer immunotherapy research.
Features:
• Enables accurate quantitation of somatic mutations to assess tumor mutation load
• Single tumor sample workflow reduces the need for a matched normal sample
• Efficient amplification from challenging samples with AmpliSeq technology
• Streamlined analysis with feature-rich visualization
• Efficient multiplexing of up to eight samples per Ion 540 Chip on the Ion S5 Systems
The Oncomine Tumor Mutation Load Assay, Chef-Ready Library Preparation, includes panel primers and reagents for automated library construction and normalization sufficient for 32 samples. This version of the assay is for automated library preparation using the Ion Chef System. For manual library preparation, please see Cat. No. A37909.
From sample to answer
The Oncomine Tumor Mutation Load Assay comes with optimized informatics and visualization software that provides a sample-to-answer solution. Run metrics can be assessed using a Torrent Suite plugin. The Ion Reporter analysis workflow utilizes a custom variant calling and germline variant filtering algorithm to accurately estimate somatic variants in cancer research samples. A detailed report is provided that includes the normalized mutation load (mutations/MB) and mutation signatures of the somatic variants, including percentage of mutations consistent with UV damage, tobacco smoke damage, de-amination, and specific substitutions.
Learn more about Oncomine Oncology ›
Features:
• Enables accurate quantitation of somatic mutations to assess tumor mutation load
• Single tumor sample workflow reduces the need for a matched normal sample
• Efficient amplification from challenging samples with AmpliSeq technology
• Streamlined analysis with feature-rich visualization
• Efficient multiplexing of up to eight samples per Ion 540 Chip on the Ion S5 Systems
The Oncomine Tumor Mutation Load Assay, Chef-Ready Library Preparation, includes panel primers and reagents for automated library construction and normalization sufficient for 32 samples. This version of the assay is for automated library preparation using the Ion Chef System. For manual library preparation, please see Cat. No. A37909.
From sample to answer
The Oncomine Tumor Mutation Load Assay comes with optimized informatics and visualization software that provides a sample-to-answer solution. Run metrics can be assessed using a Torrent Suite plugin. The Ion Reporter analysis workflow utilizes a custom variant calling and germline variant filtering algorithm to accurately estimate somatic variants in cancer research samples. A detailed report is provided that includes the normalized mutation load (mutations/MB) and mutation signatures of the somatic variants, including percentage of mutations consistent with UV damage, tobacco smoke damage, de-amination, and specific substitutions.
Learn more about Oncomine Oncology ›
Specifications
For Use With (Equipment)Ion GeneStudio S5 System, Ion S5™ XL System
LibrariesTargeted Sequencing Library
Product TypeTumor Mutation Load Assay
Quantity32 Samples
Sequencing TypeGenome & DNA Sequencing
Shipping ConditionApproved for shipment at Room Temperature or on Wet or Dry Ice
Throughput32 Samples
Workflow StepLibrary Generation
Product LineOncomine™
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• 1 Oncomine Tumor Mutation Load Panel, 32 reactions, store at -5 to -30°C.
• 4 cartridges Ion AmpliSeq Chef Reagents DL8, store at -5 to -30°C.
• 4 cartridges Ion AmpliSeq Chef Solutions DL8, store at 2–8°C.
• 4 boxes Ion AmpliSeq Supplies, store at room temperature.
• 1 IonCode in PCR Plates, store at room temperature.
• 4 cartridges Ion AmpliSeq Chef Reagents DL8, store at -5 to -30°C.
• 4 cartridges Ion AmpliSeq Chef Solutions DL8, store at 2–8°C.
• 4 boxes Ion AmpliSeq Supplies, store at room temperature.
• 1 IonCode in PCR Plates, store at room temperature.