RNAqueous™-96 Total RNA Isolation Kit
RNAqueous™-96 Total RNA Isolation Kit
Invitrogen™

RNAqueous™-96 Total RNA Isolation Kit

The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue usingRead more
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Catalog NumberQuantity
AM1920192 Preps
Catalog number AM1920
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Quantity:
192 Preps
Price (CNY)
9,728.00
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Ends: 31-Dec-2025
12,969.00
Save 3,241.00 (25%)
Each
Add to cart
The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous™-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.

Using the RNAqueous™-96 Kit
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous™-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).

Accessory product
The RNAqueous™-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume≥50 μL
Final Product TypeTotal RNA
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
For Use With (Equipment)MultiPROBE™ II HT
High-throughput CompatibilityHigh-throughput Compatible
Quantity192 Preps
Shipping ConditionBox 1: Dry Ice
Box 2: Room Temperature
Starting Material AmountCells: ≤106
Tissue: ≤1.5 mg
Isolation TechnologySilica Plate
Sample TypeCells, Tissue
Unit SizeEach
Contents & Storage
• 50 mL Nuclease-free Water; store at room temperature
• 50 mL Elution Solution; room temperature
• 2 Filter Plates; room temperature
• 2 Collection Plates; room temperature
• 2 Adhesive Plate Sealers; room temperature
• 2 Plate Lids; room temperature
• 50 mL Lysis/Binding Solution; 4°C
• 175 mL Wash Solution (concentrated); 4°C
• 53 mL Rebinding Mix; 4°C
• 12 mL DNase I Buffer; -20°C
• 1.25 mL DNase I; -20°C

Frequently asked questions (FAQs)

What are the differences in the RNAqueous kits you offer?

RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).

Can you please recommend the best manifold to use with the RNAqueous-96 Total RNA Isolation Kit (Cat. No. AM1920)?

The EveryPrep Universal Vacuum Manifold (Cat. No. K211101) is compatible with the RNAqueous-96 Total RNA Isolation Kit.