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Invitrogen™
ChargeSwitch™ Plasmid Yeast Mini Kit
Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng• Increased transformation efficiency• Reagents that avoid PCRRead more
| Catalog Number | Quantity |
|---|---|
| CS10203 | 50 Preps |
Catalog number CS10203
Price (CNY)
2,621.00
Each
Quantity:
50 Preps
Price (CNY)
2,621.00
Each
Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng
• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance
The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance
The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypePlasmid DNA
For Use With (Application)PCR, Cloning, Southern Blotting, Sequencing, Transformation
Green FeaturesLess hazardous, Fewer resources used and less waste
High-throughput CompatibilityNot High-throughput Compatible (Manual)
No. of Reactions50 Preps
Prep Scale< 100 μg (Small-Scale) Plasmid DNA
Product LineChargeSwitch™
Product TypePlasmid Yeast Mini Kit
Quantity50 Preps
Sample TypeYeast
ScaleMini
Shipping ConditionRoom Temperature
TargetPlasmid DNA
FormatKit
Isolation TechnologyChargeSwitch Chemistry
Unit SizeEach
Contents & Storage
All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store Precipitation Buffer (N5) at 4°C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.
Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Resuspension Buffer (R4)
• ChargeSwitch™ Lysis Buffer (L9)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W11)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)