ChargeSwitch™ gDNA Mini Tissue Kit

The ChargeSwitch™ gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA fromRead more

Catalog Number	Quantity
CS11204	25 Preps

Catalog number CS11204

Price (CNY)

1,554.00

Add to cart

Quantity:

25 Preps

Price (CNY)

1,554.00

Add to cart

The ChargeSwitch™ gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance

High Quality, High Yield Plasmid DNA
The ChargeSwitch™ gDNA Mini Tissue Kit allows purification of genomic DNA from 10-25 mg quantities of tissue typically in less than 15 minutes following sample preparation and lysis. ChargeSwitch™ gDNA Mini Tissue Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 30 μg of gDNA from 0.5 cm mouse tail tips or approximately 25 mg of tissue. For spleen tissue, reduce tissue size to 10 mg.

Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch™ gDNA Mini Tissue Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.

Improved Performance in Demanding Downstream Applications
The ChargeSwitch™ gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.

For Research Use Only. Not intended for diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)PCR, Southern Blotting, Sequencing, Cloning

Green FeaturesFewer resources used and less waste, less hazardous

High-throughput CompatibilityNot High-throughput Compatible (Manual)

Product LineChargeSwitch™

Product TypegDNA Mini Tissue Kit

Quantity25 Preps

Sample TypeTissue

ScaleMini

Shipping ConditionRoom Temperature

TargetGenomic DNA

Test Time15 min.

FormatKit

Isolation TechnologyChargeSwitch Chemistry

Unit SizeEach

Contents & Storage

All components of the ChargeSwitch™ gDNA Tissue Kits are shipped at room temperature. Upon receipt, store the Proteinase K and RNase A at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.

Components:
• ChargeSwitch™ Lysis Buffer (L13)
• ChargeSwitch™ Lysis Buffer (L15)
• ChargeSwitch™ Magnetic Beads
• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl₂, 50% glycerol)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Purification Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

Frequently asked questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.