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Invitrogen™
Chargeswitch™ Pro Filter Plasmid Midi Kit
The Chargeswitch™ -Pro Filter Plasmid Midi Kit includes 25 plasmid midipreps and provides high yield and high purity plasmid withRead more
| Catalog Number | Quantity |
|---|---|
| CS31104 also known as CS31104 | 25 preps |
Catalog number CS31104
also known as CS31104
Price (CNY)
7,282.00
Each
Quantity:
25 preps
Price (CNY)
7,282.00
Each
The Chargeswitch™ -Pro Filter Plasmid Midi Kit includes 25 plasmid midipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.
To accomplish this, the ChargeSwitch™-Pro Kit provides:
• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units less than 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)
How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.
The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.
To accomplish this, the ChargeSwitch™-Pro Kit provides:
• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units less than 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)
How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
Elution Volume0.5 to 1 mL
Final Product TypePlasmid DNA
For Use With (Application)Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput CompatibilityNot High-throughput Compatible (Manual)
IncludesColumns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Prep Scale100-200 μg (Medium-Scale) Plasmid DNA
Product LineChargeSwitch™
Product TypePro Filter Plasmid Midi Kit
PurityTransfection Grade
Quantity25 preps
Sample TypeBacterial Culture
ScaleMidi
Shelf LifeGuaranteed stable for 6 months when properly stored.
Shipping ConditionRoom Temperature
Starting Material Amount25 ml
System TypeChargeSwitch™
TargetPlasmid DNA
Test Time30 min.
Yield200 μg
FormatSpin Column
Isolation TechnologyChargeSwitch Chemistry
Unit SizeEach
Contents & Storage
The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.