SP-DiIC₁₈(3) (1,1'-Dioctadecyl-6,6'-Di(4-Sulfophenyl)-3,3,3',3'-Tetramethylindocarbocyanine)

SP-DiIC<sub>18</sub>(3) (1,1'-Dioctadecyl-6,6'-Di(4-Sulfophenyl)-3,3,3',3'-Tetramethylindocarbocyanine)

Citations & References (15)

Invitrogen™

SP-DiIC₁₈(3) (1,1'-Dioctadecyl-6,6'-Di(4-Sulfophenyl)-3,3,3',3'-Tetramethylindocarbocyanine)

The orange fluorescent, lipophilic carbocyanine SP-DiIC18(3) is a DiI analog that contains sulfonate groups to improve water solubility. It isRead more

Catalog Number	Quantity
D7777	5 mg

Catalog number D7777

Price (CNY)

5,805.00

Add to cart

Quantity:

Price (CNY)

5,805.00

Add to cart

The orange fluorescent, lipophilic carbocyanine SP-DiIC₁₈(3) is a DiI analog that contains sulfonate groups to improve water solubility. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (∼1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorYellow

Detection MethodFluorescence

For Use With (Equipment)Fluorescence Microscope

Quantity5 mg

Shipping ConditionRoom Temperature

Label TypeFluorescent Dye

Product TypeLiphophilic Tracer

SubCellular LocalizationCell Membranes, Lipids

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (15)

Citations & References

Abstract

Dynamic behavior of cells within neurospheres in expanding populations of neural precursors.

Authors:Wang TY, Sen A, Behie LA, Kallos MS,

Journal:Brain Res

PubMed ID:16859652

'Large-scale expansion of neural stem and progenitor cells will be essential for clinically treating the large number of patients suffering from neurodegenerative disorders such as Parkinson''s disease. Other applications of neural stem cell technology include further research in areas such as neural development or drug testing. Neural stem cells can ... More

Identification of a neural stem cell in the adult mammalian central nervous system.

Authors:Johansson CB, Momma S, Clarke DL, Risling M, Lendahl U, Frisén J

Journal:Cell

PubMed ID:9989494

'New neurons are continuously added in specific regions of the adult mammalian central nervous system. These neurons are derived from multipotent stem cells whose identity has been enigmatic. In this work, we present evidence that ependymal cells are neural stem cells. Ependymal cells give rise to a rapidly proliferating cell ... More

Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.

Authors:Daubeuf S, Bordier C, Hudrisier D, Joly E,

Journal:Cytometry A

PubMed ID:19051238

Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More

Evidence for neurogenesis in the adult mammalian substantia nigra.

Authors:Zhao M, Momma S, Delfani K, Carlen M, Cassidy RM, Johansson CB, Brismar H, Shupliakov O, Frisen J, Janson AM

Journal:Proc Natl Acad Sci U S A

PubMed ID:12792021

New neurons are generated from stem cells in a few regions of the adult mammalian brain. Here we provide evidence for the generation of dopaminergic projection neurons of the type that are lost in Parkinson's disease from stem cells in the adult rodent brain and show that the rate of ... More

Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors.

Authors:Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M

Journal:Cancer Res

PubMed ID:12097260

Molecules that physiologically control cell proliferation are often produced locally in tissues and are rapidly destroyed when they enter circulation. This allows local effects while avoiding interference with other systems. Unfortunately, it also limits the therapeutic use of these molecules via systemic delivery. We here demonstrate that, for the purpose ... More

View all SP-DiIC₁₈(3) (1,1'-Dioctadecyl-6,6'-Di(4-Sulfophenyl)-3,3,3',3'-Tetramethylindocarbocyanine) citations

SP-DiIC18(3) (1,1'-Dioctadecyl-6,6'-Di(4-Sulfophenyl)-3,3,3',3'-Tetramethylindocarbocyanine)

Frequently asked questions (FAQs)

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Citations & References (15)

SP-DiIC₁₈(3) (1,1'-Dioctadecyl-6,6'-Di(4-Sulfophenyl)-3,3,3',3'-Tetramethylindocarbocyanine)