Dynamic behavior of cells within neurospheres in expanding populations of neural precursors.
AuthorsWang TY, Sen A, Behie LA, Kallos MS,
JournalBrain Res
PubMed ID16859652
'Large-scale expansion of neural stem and progenitor cells will be essential for clinically treating the large number of patients suffering from neurodegenerative disorders such as Parkinson''s disease. Other applications of neural stem cell technology include further research in areas such as neural development or drug testing. Neural stem cells can ... More
Identification of a neural stem cell in the adult mammalian central nervous system.
AuthorsJohansson CB, Momma S, Clarke DL, Risling M, Lendahl U, Frisén J
JournalCell
PubMed ID9989494
'New neurons are continuously added in specific regions of the adult mammalian central nervous system. These neurons are derived from multipotent stem cells whose identity has been enigmatic. In this work, we present evidence that ependymal cells are neural stem cells. Ependymal cells give rise to a rapidly proliferating cell ... More
Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.
AuthorsDaubeuf S, Bordier C, Hudrisier D, Joly E,
JournalCytometry A
PubMed ID19051238
Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More
Evidence for neurogenesis in the adult mammalian substantia nigra.
AuthorsZhao M, Momma S, Delfani K, Carlen M, Cassidy RM, Johansson CB, Brismar H, Shupliakov O, Frisen J, Janson AM
JournalProc Natl Acad Sci U S A
PubMed ID12792021
New neurons are generated from stem cells in a few regions of the adult mammalian brain. Here we provide evidence for the generation of dopaminergic projection neurons of the type that are lost in Parkinson's disease from stem cells in the adult rodent brain and show that the rate of ... More
Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors.
AuthorsStudeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M
JournalCancer Res
PubMed ID12097260
Molecules that physiologically control cell proliferation are often produced locally in tissues and are rapidly destroyed when they enter circulation. This allows local effects while avoiding interference with other systems. Unfortunately, it also limits the therapeutic use of these molecules via systemic delivery. We here demonstrate that, for the purpose ... More
Agonist-induced PIP(2) hydrolysis inhibits cortical actin dynamics: regulation at a global but not at a micrometer scale.
Authorsvan Rheenen J, Jalink K
JournalMol Biol Cell
PubMed ID12221130
Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP(2) sensors suggest that this lipid is enriched in membrane microdomains. Here we report that this concept ... More
Adenovirus triggers macropinocytosis and endosomal leakage together with its clathrin-mediated uptake.
Adenovirus type 2 (Ad2) binds the coxsackie B virus Ad receptor and is endocytosed upon activation of the alphav integrin coreceptors. Here, we demonstrate that expression of dominant negative clathrin hub, eps15, or K44A-dynamin (dyn) inhibited Ad2 uptake into epithelial cells, indicating clathrin-dependent viral endocytosis. Surprisingly, Ad strongly stimulated the ... More
Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling.
AuthorsHarada H, Kettunen P, Jung HS, Mustonen T, Wang YA, Thesleff I
JournalJ Cell Biol
PubMed ID10508859
The continuously growing mouse incisor is an excellent model to analyze the mechanisms for stem cell lineage. We designed an organ culture method for the apical end of the incisor and analyzed the epithelial cell lineage by 5-bromo-2'-deoxyuridine and DiI labeling. Our results indicate that stem cells reside in the ... More
Structural microdomains in the lateral plasma membrane of cochlear outer hair cells.
AuthorsZhang M, Kalinec F
JournalJ Assoc Res Otolaryngol
PubMed ID12382104
The basal and lateral regions of the plasma membrane of cochlear outer hair cells are structurally and functionally distinct. The lateral region contains thousands of motor proteins but few voltage-gated channels. The basal region, conversely, contains a high number of voltage-gated channels but is devoid of motor proteins. It has ... More
Intracerebroventricular transplantation of ex vivo expanded endothelial colony-forming cells restores blood-brain barrier integrity and promotes angiogenesis of mice with traumatic brain injury.
Authors
JournalJ Neurotrauma
PubMed ID23957220
Wharton's jelly or bone marrow mesenchymal stromal cells improve cardiac function following myocardial infarction for more than 32 weeks in a rat model: a preliminary report.
Authors
JournalCurr Stem Cell Res Ther
PubMed ID23270633
microRNA-143/145 loss induces Ras signaling to promote aggressive Pten-deficient basal-like breast cancer.
Authors
JournalJCI Insight
PubMed ID28768903
Internalization of trophoblastic small extracellular vesicles and detection of their miRNA cargo in P-bodies.
Authors
JournalJ Extracell Vesicles
PubMed ID32944196
Abundance of d-2-hydroxyglutarate in G2/M is determined by FOXM1 in mutant IDH1-expressing cells.
Authors
JournalFEBS Lett
PubMed ID31211872
A structural and genotypic scaffold underlying temporal integration.