FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration
FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration
Invitrogen™

FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration

The FocalCheck™ fluorescence microscope test slide #1 is specifically designed for calibrating fluorescence microscope systems and evaluating system and filterRead more
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Catalog NumberQuantity
F369091 Ea.
Catalog number F36909
Price (CNY)
6,890.00
Each
Add to cart
Quantity:
1 Ea.
Price (CNY)
6,890.00
Each
Add to cart
The FocalCheck™ fluorescence microscope test slide #1 is specifically designed for calibrating fluorescence microscope systems and evaluating system and filter performance. The slide contains 10 sample areas coated with FocalCheck™ ring stained microspheres ranging in size from 0.5-15 μm that provide a visual representation of instrument misalignment or other aberrations in row A; row B contains a series of microspheres with fluorescence intensities ranging from 0.667-100%.

See our full collection of microscope calibration reagents ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibration TypeConfocal Microscope Calibration, Fluorescence Microscope Calibration
FormatSuspension Beads
Product LineFocalCheck
Quantity1 Ea.
Shipping ConditionRoom Temperature
ColorAssorted Colors
Product TypeFluorescence Microscope Test Slide
Unit SizeEach
Contents & Storage
Store at room temperature.

Frequently asked questions (FAQs)

What are the excitation/emission wavelengths for the beads in FocalCheck Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration (Cat. No. F36909)?

The spectral characteristics are as follows:

  • A1: 505/515, 560/580, 660/680
  • A2: 365/430 (core), 505/515 (ring)
  • A3-5: 365/430, 505/515, 580/600, 660/680
  • B1-5: 505/515


Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.

What type of coverslips are used for the FocalCheck Fluorescence Microscope Test Slide #1 (Cat. No. F36909)?

The coverslips are made of borosilicate glass (#1.5; ~0.17 mm thickness).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the microspheres from FocalCheck Fluorescence Microscope Test Slide #1 (Cat. No. F36909) in one plane?

No. Most of the 15 µm beads (Position A1 on the slide) would be within the narrowest focal plane, but they are not guaranteed to all be within the same plane.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (7)

Citations & References
Abstract
Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci.
Authors:Borowski LS, Dziembowski A, Hejnowicz MS, Stepien PP, Szczesny RJ,
Journal:Nucleic Acids Res
PubMed ID:23221631
RNA decay is usually mediated by protein complexes and can occur in specific foci such as P-bodies in the cytoplasm of eukaryotes. In human mitochondria nothing is known about the spatial organization of the RNA decay machinery, and the ribonuclease responsible for RNA degradation has not been identified. We demonstrate ... More
Simple, rapid zebrafish larva bioassay for assessing the potential of chemical pollutants and drugs to disrupt thyroid gland function.
Authors:Raldúa D, Babin PJ,
Journal:Environ Sci Technol
PubMed ID:19764258
Thyroid function may be altered by a very large number of chemicals routinely found in the environment Research evaluating potential thyroid disruption is ongoing, but there are thousands of synthetic and naturally occurring drugs and chemicals to be considered. European and United States policies call for the development of simple ... More
Fluorescent coherent diffractive imaging with accelerating light sheets.
Authors:Field JJ, Squier JA, Bartels RA
Journal:Opt Express
PubMed ID:31052833
'Fluorescence microscopy is a powerful method for producing high fidelity images with high spatial resolution, particularly in the biological sciences. We recently introduced coherent holographic image reconstruction by phase transfer (CHIRPT), a single-pixel imaging method that significantly improves the depth of field in fluorescence microscopy and enables holographic refocusing of ... More
IL-1ß-induced and p38
Authors:Kulawik A, Engesser R, Ehlting C, Raue A, Albrecht U, Hahn B, Lehmann WD, Gaestel M, Klingmüller U, Häussinger D, Timmer J, Bode JG
Journal:J Biol Chem
PubMed ID:28223354
The IL-1ß induced activation of the p38
Enhanced Image Sensor Module for Head-Mounted Microscopes<sup/>.
Authors:Juneau J, Duret G, Robinson J, Kemere C
Journal:Conf Proc IEEE Eng Med Biol Soc
PubMed ID:30440519
Several research groups have developed head-mounted fluorescence microscopes as a modality for recording neural activity in freely behaving mice. The current designs have shown exciting results from in vivo imaging of the bright dynamics of genetically encoded calcium indicators (GECI). However, despite their potential, head-mounted microscopes are not in use ... More