Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci.
AuthorsBorowski LS, Dziembowski A, Hejnowicz MS, Stepien PP, Szczesny RJ,
JournalNucleic Acids Res
PubMed ID23221631
RNA decay is usually mediated by protein complexes and can occur in specific foci such as P-bodies in the cytoplasm of eukaryotes. In human mitochondria nothing is known about the spatial organization of the RNA decay machinery, and the ribonuclease responsible for RNA degradation has not been identified. We demonstrate ... More
Simple, rapid zebrafish larva bioassay for assessing the potential of chemical pollutants and drugs to disrupt thyroid gland function.
AuthorsRaldúa D, Babin PJ,
JournalEnviron Sci Technol
PubMed ID19764258
Thyroid function may be altered by a very large number of chemicals routinely found in the environment Research evaluating potential thyroid disruption is ongoing, but there are thousands of synthetic and naturally occurring drugs and chemicals to be considered. European and United States policies call for the development of simple ... More
Fluorescent coherent diffractive imaging with accelerating light sheets.
AuthorsField JJ, Squier JA, Bartels RA
JournalOpt Express
PubMed ID31052833
'Fluorescence microscopy is a powerful method for producing high fidelity images with high spatial resolution, particularly in the biological sciences. We recently introduced coherent holographic image reconstruction by phase transfer (CHIRPT), a single-pixel imaging method that significantly improves the depth of field in fluorescence microscopy and enables holographic refocusing of ... More
IL-1ß-induced and p38
AuthorsKulawik A, Engesser R, Ehlting C, Raue A, Albrecht U, Hahn B, Lehmann WD, Gaestel M, Klingmüller U, Häussinger D, Timmer J, Bode JG
JournalJ Biol Chem
PubMed ID28223354
The IL-1ß induced activation of the p38
Enhanced Image Sensor Module for Head-Mounted Microscopes<sup/>.
AuthorsJuneau J, Duret G, Robinson J, Kemere C
JournalConf Proc IEEE Eng Med Biol Soc
PubMed ID30440519
Several research groups have developed head-mounted fluorescence microscopes as a modality for recording neural activity in freely behaving mice. The current designs have shown exciting results from in vivo imaging of the bright dynamics of genetically encoded calcium indicators (GECI). However, despite their potential, head-mounted microscopes are not in use ... More
Neurotransmitter diversity in pre-synaptic terminals located in the parvicellular neuroendocrine paraventricular nucleus of the rat and mouse hypothalamus.
AuthorsJohnson CS, Bains JS, Watts AG
JournalJ Comp Neurol
PubMed ID29424419
Virtually all rodent neuroendocrine corticotropin-releasing-hormone (CRH) neurons are in the dorsal medial parvicellular (mpd) part of the paraventricular nucleus of the hypothalamus (PVH). They form the final common pathway for adrenocortical stress responses. Their activity is controlled by sets of GABA-, glutamate-, and catecholamine-containing inputs arranged in an interactive pre-motor ... More
Multi-plane remote refocusing epifluorescence microscopy to image dynamic
AuthorsLawton PF, Buckley C, Saunter CD, Wilson C, Corbett AD, Salter PS, McCarron JG, Girkin JM
JournalBiomed Opt Express
PubMed ID31799034
Rapid imaging of multiple focal planes without sample movement may be achieved through remote refocusing, where imaging is carried out in a plane conjugate to the sample plane. The technique is ideally suited to studying the endothelial and smooth muscle cell layers of blood vessels. These are intrinsically linked through ... More