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Citations & References (28)
Invitrogen™
Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester)
BODIPY FL GTP-γ-S binds to GTP-binding proteins. To create this fluorescent nucleotide, the BODIPY FL fluorophore was linked through theRead more
| Catalog Number | Quantity |
|---|---|
| G22183 also known as G-22183 | 50 μL |
Catalog number G22183
also known as G-22183
Price (CNY)
8,908.00
Each
Quantity:
50 μL
Price (CNY)
8,908.00
Each
BODIPY FL GTP-γ-S binds to GTP-binding proteins. To create this fluorescent nucleotide, the BODIPY FL fluorophore was linked through the γ-thiol of GTP-γ-S. The fluorescence of BODIPY FL GTP-γ-S is quenched ∼90% relative to that of the free dye, but may be recovered on binding to G-proteins. In addition to its potential use for binding studies, the BODIPY FL GTP-γ-S thioether is an important new substrate for Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Labeling MethodChemical Labeling, Direct Labeling
Label or DyeBODIPY™ FL
Product TypeGuanosine 5'-O
Quantity50 μL
Shipping ConditionWet Ice
Concentration5 mM
Product LineBODIPY
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (28)
Citations & References
Abstract
Detection of G proteins by affinity probe capillary electrophoresis using a fluorescently labeled GTP analogue.
Journal:Anal Chem
PubMed ID:14632149
'An affinity probe capillary electrophoresis (APCE) assay for guanine-nucleotide-binding proteins (G proteins) was developed using BODIPY FL GTPgammaS (BGTPgammaS), a fluorescently labeled GTP analogue, as the affinity probe. In the assay, BGTPgammaS was incubated with samples containing G proteins and the resulting mixtures of BGTPgammaS-G protein complexes and free BGTPgammaS
Designed FHIT alleles establish that Fhit-induced apoptosis in cancer cells is limited by substrate binding.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12574506
'The FHIT gene is inactivated early in the development of many human tumors, and Fhit-deficient mice have increased cancer incidence. Viral reexpression of Fhit kills Fhit-deficient cells by induction of apoptosis. Fhit, a member of branch 2 of the histidine-triad superfamily of nucleoside monophosphate hydrolases and transferases, is a diadenosine
Fhit-nucleotide specificity probed with novel fluorescent and fluorogenic substrates.
Journal:J Biol Chem
PubMed ID:10671479
'Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins, binds and cleaves diadenosine polyphosphates and functions as a tumor suppressor in human epithelial cancers. Function of Fhit in tumor suppression does not require diadenosine polyphosphate cleavage but correlates with the ability to form substrate complexes. As diadenosine polyphosphates
Mechanism of regulation of prokaryotic tubulin-like GTPase FtsZ by membrane protein EzrA.
Journal:J Biol Chem
PubMed ID:17043359
'At initiation of cell division, FtsZ, a tubulin-like GTPase, assembles into a so-called Z-ring structure at the site of division. The formation of Z ring is negatively regulated by EzrA, which ensures only one ring at the midcell per cell cycle. The mechanism leading to the negative regulation of Z-ring
Use of a native affinity ligand for the detection of G proteins by capillary isoelectric focusing with laser-induced fluorescence detection.
Journal:Electrophoresis
PubMed ID:15274014
'Affinity probe capillary isoelectric focusing (CIEF) with laser-induced fluorescence was explored for detection of Ras-like G proteins. In the assay, a fluorescent BODIPY FL GTP analogue (BGTPgammaS) and G protein were incubated resulting in formation of BGTPgammaS-G protein complex. Excess BGTPgammaS was separated from BGTPgammaS-G protein complex by CIEF using