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Invitrogen™
pBAD202 Directional TOPO™ Expression Kit
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence ofRead more
| Catalog Number | Quantity |
|---|---|
| K420201 | 20 reactions |
Catalog number K420201
Price (CNY)
16,151.00
Each
Quantity:
20 reactions
Price (CNY)
16,151.00
Each
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.
In addition to being a more effective way to clone, TOPO™ Cloning eliminates these potential expression problems. TOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).
Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO™ cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO™ Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.
In addition to being a more effective way to clone, TOPO™ Cloning eliminates these potential expression problems. TOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).
Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO™ cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO™ Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialKanamycin (KanR)
Bacterial or Yeast StrainLMG194, TOP10
CleavageEK (Enterokinase) Recognition Site
Constitutive or Inducible SystemInducible
Expression MechanismCell-Based Expression
Expression SystemE. coli
Inducing AgentArabinose
Product TypeTOPO Expression Kit
Quantity20 reactions
Selection Agent (Eukaryotic)None
VectorpBAD
Cloning MethodDirectional TOPO
Product LineTOPO
PromoteraraBAD
Protein TagHis Tag (6x), V5 Epitope Tag, Thioredoxin
Unit SizeEach