Arcturus™ Paradise™ Plus RNA Extraction and Isolation Kit

The ARCTURUS™ Paradise™ PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples byRead more

Catalog Number	Quantity
KIT0312I	12 samples

Catalog number KIT0312I

Price (CNY)

Quantity:

12 samples

The ARCTURUS™ Paradise™ PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1).

Secure Downstream Success with the Paradise™ QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (Figure 2).

Integrated Systems for Microgenomics
The Paradise™ PLUS Reagent System is validated as part of the ARCTURUS™ complete System for Microgenomics™, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUS^XT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS™' Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product TypeTotal RNA

For Use With (Application)Microarray Analysis, Real-Time Quantitative PCR (qPCR)

For Use With (Equipment)ArcturusXT™ LCM Instrument

High-throughput CompatibilityNot High-throughput Compatible (Manual)

No. of Samples12 samples

Product LineARCTURUS™, Paradise™

Quantity12 samples

Starting Material AmountAs little as a single cell.

FormatSpin Column

Isolation TechnologyEnzymatic, Spin Column

Sample TypeLaser Capture Microdissection (LCM), Tissue (FFPE)

TargetTotal RNA

Unit SizeEach

Contents & Storage

Paradise RNA Isolation Kit: Proteinase K, Protenaise K buffer, purification reagents, purification columns, microcentrifuge tubes. Variable storage conditions

Frequently asked questions (FAQs)

What is the lowest amount of RNA that can be amplified using the Arcturus Paradise Plus RNA Extraction and Isolation Kit?

With this kit, you can amplify as little as 5 ng of RNA isolated from formalin-fixed, paraffin embedded (FFPE) tissues.

How do I locate the overview image on the Arcturus XT LCM Instrument?

First, optimize the visualization of the image in the live video at 2X by increasing or decreasing the brightness setting, then right-click on the overview image and select "remember settings". This resets your optimized image settings, and when you select "re-acquire overview image" you will now generate a perfectly exposed overview image.

With the ArcturusXT LCM Instrument, which laser should I use to isolate my region of interest? UV cutting laser or IR capture laser?

For a few cells: The gentle IR-only approach is the best recommendation for preserving nucleic acids and verifying that the desired material is collected on the cap. For groups of cells or large tumor regions: Mount your tissue on a membrane slide and take advantage of the power of the IR, or IR + UV cutting laser to cut around your region of interest quickly and cleanly.

What is Arcturus Laser Capture Microdissection (LCM)?

The ArcturusXT LCM Instrument has a proprietary combination of a gentle IR laser and a powerful UV laser that work in conjunction to efficiently isolate cells from frozen sections or paraffin embedded sections without changing morphology or integrity of the biological content. The IR laser helps to capture the cells of interest, and the UV laser microdissects the captured cells. This is done without affecting the morphology of the cells, and allows for visual inspection of the remaining specimen to help ensure the quality of the sample collected. In addition to the flexibility that the dual laser system provides, there is the flexibility of multiple stage inserts for various sample types. A wide-slide stage format is available for neurobiology researchers working with very large sections of brain tissue. The petri dish stage insert enables live-cell microdissection applications such as stem cell research and other rare-cell isolations.