NanoDrop™ Ultra dsDNA BR Fluorescence Assay Kit

The Thermo Scientific NanoDrop Ultra dsDNA BR (Broad Range) Fluorescence Assay Kit is a specialized quantification solution designed for use with the NanoDrop Ultra FL or NanoDrop UltraC FL spectrophotometer and fluorometer systems. This fluorescence-based assay provides accurate and selective quantitation of double-stranded DNA samples for qPCR and RT-PCR applications.

The assay kit delivers broad-range quantification capabilities with linear fluorescence signals spanning DNA concentrations from 10 to 1,000 ng/μL. The kit includes concentrated assay reagent, dilution buffer, and DNA standards for streamlined sample preparation. The assay protocol is optimized for rapid implementation—requiring only reagent dilution, microplate loading, sample addition, mixing, and fluorescence measurement.

The NanoDrop Ultra platform enables rapid, precise quantification of nucleic acids and proteins in single-sample workflows while ensuring effortless 21 CFR Part 11 compliance for regulated laboratory environments when needed.Features of the NanoDrop Ultra dsDNA BR Fluorescence Assay Kit include:

• Superior accuracy—delivers more precise quantification results compared to traditional UV absorbance methods by eliminating interference from free nucleotides and other sample contaminants
• Enhanced sensitivity—enables exceptional signal-to-background ratio for reliable detection and quantification of low-concentration samples
• Dilute sample quantification—maintains sensitivity and accuracy sufficient for quantitation of highly dilute biomolecule samples
• Sample preservation—enables accurate measurements while preserving valuable biomolecules for subsequent downstream applications
• Interference-free detection—minimizes measurement artifacts caused by free nucleotides and other contaminants that commonly affect UV-based quantification methods

Key performance characteristics include room-temperature operation with signal stability maintained for up to three hours post-preparation and robust tolerance to common laboratory contaminants, including salts, solvents, detergents, and proteins.

Streamlined DNA detection with minimal sample requirements

The NanoDrop Ultra dsDNA BR Fluorescence Assay represents a quick and easy kit solution designed specifically for efficient DNA detection in research samples. This fluorescence-based assay system has been engineered to provide researchers with a straightforward approach to nucleic acid quantification while maintaining the precision standards required for advanced scientific applications.

Seamless integration with NanoDrop Ultra technology

The assay kit operates through direct compatibility with NanoDrop Ultra instrumentation, leveraging the established microvolume spectrophotometry platform that has become standard in molecular biology laboratories. This technological integration ensures that researchers can utilize their existing NanoDrop Ultra systems to perform fluorescence-based DNA quantification without requiring additional specialized equipment or extensive protocol modifications.

Precision quantification with enhanced sample conservation

The NanoDrop Ultra dsDNA BR Fluorescence Assay is specifically engineered for researchers who require precise DNA quantification while minimizing sample consumption. The broad range fluorescence methodology enables accurate detection across a wide concentration spectrum, making it particularly valuable for applications where sample availability is limited or where conservation of precious biological material is paramount. This combination of precision and sample efficiency addresses critical needs in contemporary molecular biology research where both accuracy and resource optimization are essential considerations.