NanoDrop™ Ultra RNA BR Fluorescence Assay Kit

The Thermo Scientific NanoDrop Ultra RNA BR Fluorescence Assay is a specialized quantitation solution engineered for precise and selective measurement of total RNA, ribosomal RNA (rRNA), and large messenger RNA (mRNA) species. This fluorescence-based assay system is specifically designed for compatibility with NanoDrop Ultra FL and UltraC FL spectrophotometers, delivering reliable quantitative analysis across a broad dynamic range of 10 to 1,000 ng/μL RNA concentrations.

The assay leverages advanced fluorescence detection technology to provide linear signal response while maintaining exceptional tolerance to common laboratory contaminants, including salts, solvents, detergents, and proteins. The complete system includes concentrated assay reagent, dilution buffer, and RNA standards, enabling streamlined sample preparation through a simple dilute-load-mix-read protocol. Measurements are performed at ambient temperature with signal stability maintained for up to three hours, facilitating flexible workflow integration.

This fluorescence assay represents a key component of the NanoDrop Ultra platform's multimode detection capabilities, offering researchers enhanced sensitivity and precision for nucleic acid quantification workflows, particularly those involving quantitative PCR (qPCR) and reverse transcription PCR (RT-PCR) applications. The system provides microliter-scale analysis with 21 CFR Part 11 compliance capabilities, supporting both research and regulated laboratory environments.

Features of the NanoDrop Ultra RNA BR Fluorescence Assay include:

• Superior accuracy—delivers more precise measurements than UV absorbance readings by eliminating interference from free nucleotides and other contaminants that compromise traditional spectrophotometric methods
• Enhanced sensitivity—provides exceptional signal-to-background ratio, enabling reliable quantification of low-concentration samples where conventional methods may fail
• Sample preservation—maintains sufficient sensitivity and accuracy to quantitate highly dilute samples, minimizing sample consumption and preserving valuable biomolecules for critical downstream applications
• Contaminant-free detection—eliminates measurement artifacts caused by free nucleotides and other interfering substances commonly present in biological samples
• Low-concentration capability—optimized for accurate quantification of samples at the lower limits of detection, expanding the range of measurable sample types

Precision RNA detection through innovative fluorescence technology

The NanoDrop Ultra RNA BR Fluorescence Assay represents a significant advancement in nucleic acid quantification methodology, specifically engineered for NanoDrop Ultra RNA detection applications. This sophisticated assay leverages cutting-edge fluorescence-based detection principles to deliver highly accurate RNA measurements while maintaining the operational simplicity that researchers require in demanding laboratory environments. The technology underlying this assay has been optimized to provide reliable quantification across a broad range of RNA concentrations, making it suitable for diverse research applications where precision is paramount.

Streamlined single-kit solution for rapid RNA identification

The assay's design philosophy centers on operational efficiency, offering researchers a comprehensive single kit solution that enables quick identification of RNA samples without the complexity of multiple reagent systems. This unified approach significantly reduces preparation time and minimizes the potential for procedural errors that can occur when coordinating multiple components. The streamlined protocol allows researchers to transition seamlessly from sample preparation to results acquisition, thereby accelerating research timelines while maintaining rigorous analytical standards.

Optimized for sample conservation and research efficiency

The NanoDrop Ultra RNA BR Fluorescence Assay addresses a critical concern in molecular biology research by minimizing sample waste during the quantification process. This feature proves particularly valuable when working with precious or limited RNA samples, such as those derived from rare clinical specimens or challenging extraction procedures. The assay's efficiency in sample utilization allows researchers to preserve valuable material for downstream applications while still obtaining the precise quantification data necessary for experimental planning. This combination of accuracy and sample conservation makes the assay a superior choice for researchers who require dependable RNA quantification without compromising their sample integrity or availability for subsequent analyses.