EDTA (0.5 M), pH 8.0

Thermo Scientific 0.5 M EDTA, pH 8.0 is an aqueous solution of 0.22 μm membrane-filtered ethylendiaminetetraacetic acid (EDTA), sodium salt. It's ideal for biochemistry or molecular biology applications that require a chelator for divalent cations. By forming complexes with metal ions, EDTA inhibits metal-dependent enzymatic reactions. EDTA complexes metal ions at a 1:1 molar ratio.

Nucleic acids are normally stored in TE buffer that contains 1 mM EDTA, which prevents degradation of nucleic acids by metal dependent nucleases.

Applications

• Termination of metal dependent enzymatic reactions
• Protection of DNA against metal dependent nucleases