Quant-it™ RiboGreen Reagent and RNA Assay Kit
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Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
For accurate quantification and a streamlined workflow try our new Quant-iT RiboGreen ReadyPlates!
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Invitrogen™

Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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Catalog NumberQuantityProduct TypeQuantitation Range
R114911 mLQuant-iT RiboGreen RNA Reagent1 to 200 ng
R114901 kitQuant-iT RiboGreen RNA Kit1 to 200 ng
R327001 kitRediPlate 96 RiboGreen RNA Kit3 to 200 ng
Catalog number R11491
Price (CNY)
7,293.00
Each
Add to cart
Quantity:
1 mL
Product Type:
Quant-iT RiboGreen RNA Reagent
Quantitation Range:
1 to 200 ng
Price (CNY)
7,293.00
Each
Add to cart
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

Workflow 

This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection.  To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL.  RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.

Applications

Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200-2,000 cuvette assays using a 2 mL assay volume
Product LineQuant-iT, RIBOGREEN
Product TypeQuant-iT RiboGreen RNA Reagent
Quantitation Range1 to 200 ng
Quantity1 mL
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

What are the linear ranges of the Quant-iT RNA kits?

The linear ranges are:

- Quant-iT RiboGreen RNA Assay: 1 ng/mL to 1 µg/mL
- Quant-iT RNA BR Assay: 20-1,000 ng
- Quant-iT RNA HS Assay: 5-100 ng

What is the difference between the Quant-iT RNA assay and the Quant-iT RiboGreen RNA Assay?

There are several differences:

- The Quant-iT RNA Assay uses a far-red dye (excitation 644 nm/emission 673 nm); the Quant-iT RiboGreen RNA assay uses a green fluorescent dye (excitation 500 nm/emission 525 nm).
- The Quant-iT RNA assay is more specific for RNA.
- Quant-iT RNA Assays are less sensitive than the Quant-iT RiboGreen RNA Assay.

Can I make my own assay for the Qubit Fluorometer?

Yes, you can, for Qubit instruments developed after the original Qubit (1.0) Fluorometer. See MyQubit assay instructions here (http://www.thermofisher.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-assays/myqubit.html.html).

I have a crude lysate. Will the Quant-iT and Qubit assays work?

Generally, the cleaner the sample the better. Some salts, proteins, and detergents are tolerated in the assays; see the specific assay protocol for which ones and at what concentrations.

View all Quant-it™ RiboGreen Reagent and RNA Assay Kit FAQs