SYTO™ RNASelect™ Red

SYTO™ RNASelect™ is a bright photostable cell-permeant, RNA-selective fluorescent stain for imaging endogenous RNA organization in live or fixed eukaryotic cells. This molecular probe is a ready-to-use 100x aqueous solution to add directly to media or buffer to label cells.

Red-channel detection supports Texas Red filter sets, helping reserve GFP/FITC channels for antibodies, fluorescent proteins, or other structural markers.

Compatibility with 4% paraformaldehyde-based workflows and with downstream permeabilization or immunofluorescence workflows expands applications into high content screening for nucleolar reorganization, cell stress assays, and deep learning computational models.

Staining is with high-affinity with RNA specificity, with additional nuclear and cytoplasmic RNA-associated signal for visualization of RNA-rich cellular compartments.

RNase-responsive staining pattern supports use as an RNA-associated imaging readout, and can be multiplexed with other nucleic acid labels.

Features

• Suitable for live-to-fixed workflows, connecting live-cell observation with endpoint imaging.
• Compatible with multiplex imaging when paired with spectrally separated markers such as Hoechst/DAPI, phalloidin, ER, or mitochondrial probes.
• Applicable to high-content imaging and RNA-focused morphology studies, including workflows where nucleolar signal is used for segmentation or comparative phenotyping.

For researchers familiar with SYTO™ 14, SYTO™ RNASelect™ Red offers a red-channel option for RNA-associated morphology in Cell Painting-related or other multiplex imaging workflows.

Complements SYTO™ RNASelect™ Green in channel planning and fixation choice: SYTO™ RNASelect™ Green remains the better fit when methanol fixation is required, whereas SYTO™ RNASelect™ Red is appropriate when a red RNA channel or 4% PFA workflow is preferred.

Available as S32710 and in a 3-pack format as S32711.