ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit
ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit
Citations & References (6)
Invitrogen™

ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit

Achieve unparalleled precision and sensitivity in your fluorescence-based applications from FISH to real-time PCR. With a streamlined, non-enzymatic labeling process and a broad selection of vibrant dyes, ULYSIS kits ensure your nucleic acids are ready for any challenge.
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Catalog NumberLabel or DyeExcitation/Emission
U21660Alexa Fluor 647650/670 nm
U21654Alexa Fluor 594588/615 nm
Catalog number U21660
Price (CNY)
8,795.00
1 kit
Add to cart
Label or Dye:
Alexa Fluor 647
Excitation/Emission:
650/670 nm
Price (CNY)
8,795.00
1 kit
Add to cart

ULYSIS nucleic acid labeling kits provide a unique method for attaching a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast - just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.

Features include:

  • Labeling reaction complete in as little as 15 minutes
  • Available in several Alexa Fluor dye colors
  • Non-enzymatic labeling—avoids the potential biases and limitations associated with enzymatic labeling methods
  • Flexibility—compatible with a wide range of experimental conditions and protocols
  • High sensitivity—provides strong fluorescence signals, enabling detection of low-abundance targets

Reliable labeling with the Universal Linkage System

This series of ULYSIS kits was developed to enable rapid and simple coupling of Alexa Fluor dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), makes use of a platinum dye complex that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.

Labeling is fast and easy

The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS complex can be accomplished with a simple spin-column procedure. DNA longer than ∼1,000 base pairs require a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorFar-red
Excitation/Emission650/670 nm
For Use With (Application)Dot blot, Northern blot, Southern blot, RNA in situ hybridization, DNA in situ hybridization, Multicolor fluorescence in situ hybridization (mFISH), Comparative genome hybridization (CGH), Microarray analysis
Includes Label or DyeYes
Labeling MethodDirect Labeling
Product LineAlexa Fluor, Ulysis
Product TypeNucleic Acid Labeling Kit
Quantity1 Kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Final Product TypeProbes (Labeled RNA), Probes (Labeled DNA), Oligos (Labeled)
FormatKit
Labeling TargetDNA (General), Oligos, RNA (General)
Label or DyeAlexa Fluor 647
Sample TypeDNA/RNA
Unit Size1 kit
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Needed but not included: gel filtration-based spin columns for purification of the labeled DNA from excess labeling reagent

Frequently asked questions (FAQs)

Is ULYSIS labeling compatible with microarray analysis?

Yes, there are numerous examples of ULYSIS labeled probes that have been used in microarray analysis. Here are a few publications for your reference:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can probes labeled with the ULYSIS Nucleic Acid Labeling Kits be stored for later use?

Long-term storage for the ULYSIS labeled probes can be done in just about any kind of buffer, TE, formamide, hybridization buffer, or ethanol. We suggest using your normal storage conditions as long as you protect the probes from light. ULYSIS conjugates are very stable. Avoid phenol.

Do you have any tips on using the ULYSIS Nucleic Acid Labeling Kits for RNA labeling?

A preliminary protocol modifies our DNA-labeling protocol: Do not nuclease-treat the RNA, but label it directly by incubating for 10 minutes at 90°C or 15 minutes at 85°C. Add 2 µg of glycogen for every 1 µg of RNA and purify by ethanol precipitation. Refer to these publications:

- Babak T, Zhang W, Marros Q et al. (2004) Probing microRNAs with microarrays: tissue specificity and functional inference. RNA 10(11):1813-1819.
- Hiley SL, Jackman J, Babak T et al. (2005) Detection and discovery of RNA modifications using microarrays. Nucleic Acids Res 33(1):e2.
- Torchet C, Badis G, Devaux F et al. (2005) The complete set of H/ACA snoRNAs that guide rRNA pseudouridylations in Saccharomyces cerevisiae. RNA 11(6):928-938.

Can the ULYSIS kits be used on probes longer than 1,000 base pairs or even plasmids?

It might be possible to label larger probes with the ULYSIS Nucleic Acid Labeling Kits, but the dye will likely need to be diluted to avoid (or at least reduce) problems with aggregation. Refer to this publication: Coelho-Castelo AA, Santos Junior RR, Bonato VL et al. (2003) B-lymphocytes in bone marrow or lymph nodes can take up plasmid DNA after intramuscular delivery. Hum Gene Ther 14(13):1279-1285.

How stable is the ULYSIS labeled DNA to high temperature?

An oligonucleotide labeled with a ULYSIS Nucleic Acid Labeling Kit should survive 100°C for 5 minutes, and storage at 68°C overnight should also not cause any dissociation of the complex.

View all ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit FAQs

Citations & References (6)

Citations & References
Abstract
A systematic search for new mammalian noncoding RNAs indicates little conserved intergenic transcription.
Authors:Babak T, Blencowe BJ, Hughes TR
Journal:BMC Genomics
PubMed ID:16083503
'BACKGROUND: Systematic identification and functional characterization of novel types of noncoding (nc)RNA in genomes is more difficult than it is for protein coding mRNAs, since ncRNAs typically do not possess sequence features such as splicing or translation signals, or long open reading frames. Recent "tiling" microarray studies have reported that ... More
A custom microarray platform for analysis of microRNA gene expression.
Authors:Thomson JM, Parker J, Perou CM, Hammond SM
Journal:Nat Methods
PubMed ID:15782152
'MicroRNAs are short, noncoding RNA transcripts that post-transcriptionally regulate gene expression. Several hundred microRNA genes have been identified in Caenorhabditis elegans, Drosophila, plants and mammals. MicroRNAs have been linked to developmental processes in C. elegans, plants and humans and to cell growth and apoptosis in Drosophila. A major impediment in ... More
Expression profile analysis of microRNA (miRNA) in mouse central nervous system using a new miRNA detection system that examines hybridization signals at every step of washing.
Authors:Hohjoh H, Fukushima T
Journal:Gene
PubMed ID:17229533
'MicroRNAs (miRNAs) are small noncoding RNAs, with a length of 19 to 23 nucleotides, which appear to be involved in the regulation of gene expression by inhibiting the translation of messenger RNA. Expression profile analysis of miRNAs is necessary to understand their complex role in the regulation of gene expression ... More
Identification and characterization of bacterial pathogens causing bloodstream infections by DNA microarray.
Authors:Cleven BE, Palka-Santini M, Gielen J, Meembor S, Krönke M, Krut O
Journal:J Clin Microbiol
PubMed ID:16825354
'Bloodstream infections are potentially life-threatening and require rapid identification and antibiotic susceptibility testing of the causative pathogen in order to facilitate specific antimicrobial therapy. We developed a prototype DNA microarray for the identification and characterization of three important bacteremia-causing species: Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The array consisted ... More
Probing microRNAs with microarrays: tissue specificity and functional inference.
Authors:Babak T, Zhang W, Morris Q, Blencowe BJ, Hughes TR
Journal:RNA
PubMed ID:15496526
MicroRNAs (miRNAs) are short, stable, noncoding RNAs involved in post-transcriptional gene silencing via hybridization to mRNA. Few have been thoroughly characterized in any species. Here, we describe a method to detect miRNAs using micro-arrays, in which the miRNAs are directly hybridized to the array. We used this method to analyze ... More
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