A systematic search for new mammalian noncoding RNAs indicates little conserved intergenic transcription.
AuthorsBabak T, Blencowe BJ, Hughes TR
JournalBMC Genomics
PubMed ID16083503
'BACKGROUND: Systematic identification and functional characterization of novel types of noncoding (nc)RNA in genomes is more difficult than it is for protein coding mRNAs, since ncRNAs typically do not possess sequence features such as splicing or translation signals, or long open reading frames. Recent "tiling" microarray studies have reported that ... More
A custom microarray platform for analysis of microRNA gene expression.
AuthorsThomson JM, Parker J, Perou CM, Hammond SM
JournalNat Methods
PubMed ID15782152
'MicroRNAs are short, noncoding RNA transcripts that post-transcriptionally regulate gene expression. Several hundred microRNA genes have been identified in Caenorhabditis elegans, Drosophila, plants and mammals. MicroRNAs have been linked to developmental processes in C. elegans, plants and humans and to cell growth and apoptosis in Drosophila. A major impediment in ... More
Expression profile analysis of microRNA (miRNA) in mouse central nervous system using a new miRNA detection system that examines hybridization signals at every step of washing.
AuthorsHohjoh H, Fukushima T
JournalGene
PubMed ID17229533
'MicroRNAs (miRNAs) are small noncoding RNAs, with a length of 19 to 23 nucleotides, which appear to be involved in the regulation of gene expression by inhibiting the translation of messenger RNA. Expression profile analysis of miRNAs is necessary to understand their complex role in the regulation of gene expression ... More
Identification and characterization of bacterial pathogens causing bloodstream infections by DNA microarray.
AuthorsCleven BE, Palka-Santini M, Gielen J, Meembor S, Krönke M, Krut O
JournalJ Clin Microbiol
PubMed ID16825354
'Bloodstream infections are potentially life-threatening and require rapid identification and antibiotic susceptibility testing of the causative pathogen in order to facilitate specific antimicrobial therapy. We developed a prototype DNA microarray for the identification and characterization of three important bacteremia-causing species: Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The array consisted ... More
Probing microRNAs with microarrays: tissue specificity and functional inference.
AuthorsBabak T, Zhang W, Morris Q, Blencowe BJ, Hughes TR
JournalRNA
PubMed ID15496526
MicroRNAs (miRNAs) are short, stable, noncoding RNAs involved in post-transcriptional gene silencing via hybridization to mRNA. Few have been thoroughly characterized in any species. Here, we describe a method to detect miRNAs using micro-arrays, in which the miRNAs are directly hybridized to the array. We used this method to analyze ... More
The clustering of four subfamilies of satellite DNA at individual chromosome ends in Silene latifolia.
AuthorsKazama Y, Sugiyama R, Suto Y, Uchida W, Kawano S
JournalGenome
PubMed ID16767177
The satellite DNA (satDNA) on the ends of chromosomes has been isolated and characterized in the dioecious plant Silene latifolia. BAC clones containing large numbers of repeat units of satDNA in a tandem array were isolated to examine the clustering of the repeat units. satDNA repeat units were purified from ... More