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Supporting assay development, validation, and molecular testing
Applied Biosystems TaqPath master mixes combine reproducible analytical performance, inhibitor tolerance, and compliance support for assay development, validation, quality control, and molecular testing applications. Designed to help meet the needs of diagnostic developers, testing laboratories, and biopharma organizations, TaqPath master mixes support consistent results across a broad range of qPCR and RT-qPCR applications.
Performance across challenging qPCR and RT-qPCR applications
Whether detecting low-level targets, quantifying gene expression, or working with challenging sample matrices, TaqPath master mixes are designed to enable the sensitivity, precision, and reproducibility required across a broad range of qPCR and RT-qPCR applications.
* Data shown are illustrative of product performance. Results may vary depending on assay design, target, sample type, instrument, and workflow.
Quality systems, compliance support, and supply continuity
TaqPath qPCR and RT-qPCR master mixes are supported by robust analytical and functional quality control designed to help ensure lot-to-lot consistency and reproducible performance. Manufactured in an ISO 13485-certified, FDA-registered facility that adheres to cGMP principles, TaqPath master mixes are supported by accessible compliance documentation, change notification support, and dual-site manufacturing to help support continuity from assay development through routine testing
Which TaqPath master mix should I use?
TaqPath master mixes are optimized for specific applications and workflows. Start by identifying your application, such as gene expression analysis, microbial detection, or multiplex RT-qPCR, then use the guide below to select the chemistry and capabilities best suited to your workflow requirements.
Application |
Recommended product |
Key capabilities |
|---|---|---|
2-step gene expression |
Sensitive quantification, multiplex gene expression |
|
1-step gene expression |
Higher order multiplexing, benchtop stability, automation compatibility, inhibitor tolerance |
|
Microbial detection |
TaqPath BactoPure Microbial Detection Master Mix or TaqPath DuraPlex 1-Step RT-qPCR Master Mix |
Inhibitor tolerance, reliable target detection, challenging sample matrices, multiplexing |
SNP genotyping |
Accurate genotyping, multiplexing, inhibitor tolerance, crude lysate compatibility | |
Copy number variation analysis |
Accurate copy number determination, reproducible genomic DNA analysis |
TaqPath master mixes
Need OEM or custom solutions?
Need support beyond off-the-shelf reagents? Thermo Fisher Scientific offers OEM support, custom manufacturing, and commercialization services to help support diagnostic, molecular testing, and biopharma programs.
Frequently asked questions about TaqPath Master Mixes
Choose TaqPath qPCR Master Mix, CG for 2-step gene expression workflows where reverse transcription is performed separately from qPCR. Choose TaqPath DuraPlex 1-Step RT-qPCR Master Mix for 1-step RT-qPCR workflows where reverse transcription and qPCR are performed in a single reaction. The appropriate choice depends on your workflow design, multiplexing requirements, and sample type.
For DNA-based microbial detection workflows, choose TaqPath BactoPure Microbial Detection Master Mix. It is designed for microbial detection applications and inhibitor-rich sample matrices. For workflows requiring RNA detection or a 1-step RT-qPCR workflow, consider TaqPath DuraPlex 1-Step RT-qPCR Master Mix.
If your workflow requires detection of multiple targets in a single RT-qPCR reaction, consider TaqPath DuraPlex 1-Step RT-qPCR Master Mix. It is designed for higher-order multiplexing and supports automated and high-throughput workflows.
Choose TaqPath ProAmp Master Mix for SNP genotyping and CNV analysis workflows. It is designed for genomic DNA applications and supports compatibility with crude lysates, inhibitor tolerance, and high-throughput workflows.
TaqPath master mixes are compatible with a broad range of qPCR instruments. They have been evaluated on Applied Biosystems, Roche, and Bio-Rad real-time PCR systems. Refer to individual product pages for product-specific instrument compatibility information and workflow recommendations.
Choose TaqPath qPCR Master Mix, CG for 2-step gene expression workflows where reverse transcription is performed separately from qPCR. Choose TaqPath DuraPlex 1-Step RT-qPCR Master Mix when reverse transcription and qPCR are performed in a single reaction. The choice depends on your workflow design, sample type, and multiplexing requirements.
TaqPath master mixes can be evaluated using your own assays, samples, and qPCR instruments to assess performance under your workflow conditions. Depending on your region and product availability, evaluation options may be available. Technical support resources and workflow guidance are available throughout the evaluation process to support optimization and implementation.
TaqPath master mixes are supported by compliance documentation and change notification support to help simplify regulated workflow management. TaqPath reagents are manufactured in an ISO 13485-certified, FDA-registered facility that adheres to cGMP principles and are supported by analytical and functional quality control processes designed to help ensure lot-to-lot consistency and reproducible performance. Refer to individual product pages for additional information.
Order TaqPath Master Mixes
TaqPath Duraplex 1-Step RT-qPCR Master Mix
TaqPath Bactopure Microbial Detection Master Mixes
TaqPath qPCR Master Mix, CG
TaqPath ProAmp Master Mixes
TaqPath master mixes are general purpose reagents labeled ‘For Laboratory Use’ and manufactured in an ISO 13485-certified and FDA-registered facility that adheres to cGMP principles.