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Invitrogen
CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), PE, eBioscience™
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-Flow.jpg?time=20240507 "CD90.1 (Thy-1.1) Antibody in Flow Cytometry (Flow)")
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CD90.1 (Thy-1.1) Antibody (12-0900-81) in Flow
Staining of rat splenocytes with Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (left, open histogram) or Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (right, open histogram) and Anti-Mouse/Rat CD90.1 (Thy-1.1) FITC (Product # 11-0902-82) (left, filled histogram) or 0.03 µg of Anti-Mouse/Rat CD90.1 (Thy-1.1) PE (right, filled histogram). Total viable cells were used for analysi... View More
-Flow.jpg?time=20240507)
-Flow.jpg?time=20240507?time=20240507 "CD90.1 (Thy-1.1) Antibody (12-0900-81) in Flow")
产品信息
12-0900-81
产品规格
种属反应
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
HIS51
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.
Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; thymus cell antigen 1, theta; Thymus cell surface antigen
基因别名: CD7; CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2
UniProt ID: (Mouse) P01831, (Rat) P01830
Entrez Gene ID: (Mouse) 21838, (Rat) 24832
angiogenesis
negative regulation of protein kinase activity
cytoskeleton organization
single organismal cell-cell adhesion
negative regulation of cell migration
positive regulation of GTPase activity
retinal cone cell development
protein autophosphorylation
focal adhesion assembly
negative regulation of axonogenesis
T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of release of sequestered calcium ion into cytosol
negative regulation of apoptotic process
mast cell activation
negative regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
