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Invitrogen
CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Super Bright™ 645, eBioscience™
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FIGURE: 1 / 20
CD90.1 (Thy-1.1) Antibody (64-0900-82) in Flow
Staining of rat splenocytes with Anti-Rat CD3 APC (Product # 17-0030) and 0.25 µg of Mouse IgG2a kappa Isotype Control Super Bright 645 (Product # 64-4724) (left) or 0.25 µg of CD90-1 (Thy-1-1) Monoclonal Antibody, Super Bright 645 (right). Total viable cells were used for analysis.
Product Details
64-0900-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HIS51
Conjugate
Excitation/Emission Max
414/645 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.
Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; thymus cell antigen 1, theta; Thymus cell surface antigen
Gene Aliases: CD7; CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2
UniProt ID: (Mouse) P01831, (Rat) P01830
Entrez Gene ID: (Mouse) 21838, (Rat) 24832
angiogenesis
negative regulation of protein kinase activity
cytoskeleton organization
single organismal cell-cell adhesion
negative regulation of cell migration
positive regulation of GTPase activity
retinal cone cell development
protein autophosphorylation
focal adhesion assembly
negative regulation of axonogenesis
T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of release of sequestered calcium ion into cytosol
negative regulation of apoptotic process
mast cell activation
negative regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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