Cells-to-CT™ 散装 Fast Advanced RT 试剂
Cells-to-CT™ 散装 Fast Advanced RT 试剂
Invitrogen™

Cells-to-CT™ 散装 Fast Advanced RT 试剂

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Invitrogen Fast Advanced Cells-to-CT 散装 RT 试剂可单独提供大容量版本的逆转录（RT）试剂用于 TaqMan了解更多信息
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货号数量
A391102500 reactions
货号 A39110
价格（CNY)
83,884.00
Each
添加至购物车
数量:
2500 reactions
价格（CNY)
83,884.00
Each
添加至购物车
Invitrogen Fast Advanced Cells-to-CT 散装 RT 试剂可单独提供大容量版本的逆转录（RT）试剂用于 TaqMan Fast Advanced Cells-to-CT 试剂盒和 SYBR Green Fast Advanced Cells-to-CT 试剂盒 （20 X RT 酶混合物和20 X RT 缓冲液）。这些试剂足够用于 2500 次 RT 反应。与 Cells-to-CT 散装裂解试剂结合使用时，这些试剂可轻松整合至自动化的高通量应用中。
仅供科研使用。不可用于诊断程序。
规格
保真度（相对于 Taq）20X
环保功能所用资源更少、废弃物更少、危害更小
高通量能力兼容高通量应用
反应次数2500 次反应
产品线Cells-to-CT™
产品类型散装 Fast Advanced Rt 试剂
纯化目标裂解物
数量2500 reactions
样品类型Cell
运输条件干冰
足够用于2500 次反应
容量68 mL
最大浓度20X
检测方法引物-探针
适用于（应用）Gene Expression
高 GC PCR 扩增效果高
反应速度快速
Unit SizeEach
内容与储存
• 6.8 mL 20X Fast Advanced RT 酶混合物
• 68 mL 2X Fast Advanced RT 缓冲液

在 -5° 至 -30°C 下储存。

常见问题解答 (FAQ)

我的特定细胞系能用Cells-to-CT系统吗？

Cells-to-CT系统应该能适用于所有细胞系。但是，由于细胞大小和成分的差异，每个裂解反应的最大细胞数在不同细胞系中会有轻微差异。您可使用TaqMan对照试剂盒检测抑制和最小样品起始量。

Will the Cells-to-CT system work with my special cell line?

There is no reason why the Cells-to-CT system shouldn’t work with any cell line. However, due to differences in cell size and composition, the maximum number of cells per lysis reaction may be slightly different for different cell lines. We recommend testing for inhibition and optimal cell input by using the TaqMan Cells-to-CT and SYBR Green Cells-to-CT Control kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the size of the cDNA produced by the reverse transcriptase in the Cells-to-CT Bulk Fast Advanced RT Reagents?

The reverse transcriptase in this kit can make cDNA up to 7 Kb. However, this was not specifically tested on Cells-to-Ct lysates.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

Do the 2-step Cells-to-CT kits contain RNase inhibitor?

Yes, the Stop Solution provided in the 2-step Cells-to-CT kits contains RNase inhibitor.

Can I use the TaqMan Gene Expression Cells-to-CT kit for multiplexing?

The TaqMan Gene Expression Cells-to-CT kit has been validated for duplexing. If you want to set up a multiplex real-time PCR reaction with 3 assays, we recommend using the TaqMan Fast Advanced Cells-to-CT kit (https://www.thermofisher.com/order/catalog/product/A35374).

View all Cells-to-CT™ 散装 Fast Advanced RT 试剂 FAQs