Invitrogen™

SYBR™ Green Fast Advanced Cells-to-CT™ 试剂盒

Name: SYBR™ Green Fast Advanced Cells-to-CT™ 试剂盒
SKU: A35381
Price: 71655.00 CNY

SYBR Green Fast Advanced Cells-to-CT 试剂盒是一个完整的细胞裂解物系统，可直接根据培养细胞进行基因表达分析，而无需 RNA 纯化步骤。

更改视图

货号	反应次数	脱氧核糖核酸酶
A35381	400 次反应	220 μL DNase I
A35379	40 次反应	22 μL DNase I
A35380	100 次反应	55 μL DNase I

3 选项

货号 A35381

价格（CNY)

71,655.00

Each

反应次数:

400 次反应

脱氧核糖核酸酶:

220 μL DNase I

价格（CNY)

71,655.00

Each

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. SYBR Green Fast Advanced Cells-to-Ct cell lysis reagents are supplied with highest sensitivity Cells-to-Ct Fast Advanced reverse transcription enzyme mix, 2X RT buffer, and best-in-class PowerUp SYBR Green Master mix. The SYBR Green Fast Advanced Cells-to-Ct Kit gives you the sensitivity you need in the fastest workflow available: get gene expressions analysis results from an entire plate of cells in about 1.5 hours.

Complete – optimized workflow includes cell lysis reagents with gDNA removal, new Fast Advanced RT enzyme mix, buffer, and new PowerUp SYBR Green Fast Advanced Master Mix
Fast – 7-minute sample prep, including DNase treatment, at room temperature
Easy – lyse samples in a tube or directly in culture plates
Robust – perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
Efficient – contains sufficient reagents to generate 1000 real-time PCR results from 100 starting samples

SYBR Green Fast Advanced Cells-to-CT 试剂盒是一个完整的细胞裂解物系统，可直接根据培养细胞进行基因表达分析，而无需 RNA 纯化步骤。SYBR Green Fast Advanced Cells-to-CT 细胞裂解试剂提供了最高灵敏度的 Cells-to-CT Fast Advanced 逆转录酶混合物、2X RT 缓冲液和一流的 PowerUp SYBR Green预混液。SYBR Green Fast Advanced Cells-to-CT 试剂盒可在现有最快的工作流程中为您提供您所需的灵敏度：在约1.5小时内从整个细胞板中获得基因表达分析结果。

•全面—优化的工作流程包括移除 gDNA 的细胞裂解试剂、新型 Fast Advanced RT 酶混合物、缓冲液和新型 PowerUp SYBR Green Fast Advanced 预混液
•快速—7分钟样品制备，包括在室温下进行 DNase处理
•简便—可以在试管或直接在培养板中裂解样品
•稳健—可以对每个样品10–100,000个细胞进行基因表达分析；结果与从纯化 RNA 中获得的等效
•高效—含有足够的试剂以从100起始样品中生成1000实时荧光定量 PCR 结果

无需从培养细胞中分离 RNA 的独特方法
Cells-to-Ct 方法具有独特的裂解试剂，可裂解细胞并保护 RNA。5在分钟裂解步骤中同时去除 DNA，可节省时间并提高易用性。然后通过加入“终止液停止裂解，”该终止液会不可逆地与裂解试剂结合。

SYBR Green Fast Advanced Cells-to-CT 试剂盒含有用于 cDNA 合成的 Cells-to-Ct Fast Advanced 逆转录 (RT) 试剂和用于实时荧光定量 PCR 分析的 PowerUp SYBR Green预混液。引物对由用户提供。

简单7-分钟的样品制备
SYBR Green Fast Advanced Cells-to-CT 试剂10–盒专门设计用于100,000个培养细胞/样品。细胞先用 PBS 清洗，再于室温下置于裂解液中裂解5分钟；同时进行 DNase 处理。裂解过程在加入终止液，室温孵育 2 分钟后终止。裂解液现可直接进行逆转录或在–20°C条件下短期储存，或在-80C

条件下长期储存。较高的检测灵敏度
不同于某些将 RT 反应中裂解物的量限制在10%的竞品试剂盒，SYBR Green Fast Advanced Cells-to-CT 试剂盒可以容纳总 RT 反应体积45%的细胞溶解产物。此外，cDNA 至多可占实时荧光定量 PCR 反应体积的30%。在优化的 RT 反应中加入大量裂解物，以及后续使用 PowerUP SYBR Green 预混液进行实时荧光定量 PCR 反应的大量 cDNA ，可较大限度提高灵敏度。该预混液可精确而准确地对靶标进行扩增，从而能够检测少量靶标，如低表达水平的转录物。。

减少样品损失和转移错误
由于可直接在培养孔（96或384孔）中处理样品，故样品处理和样品损失或转移错误的可能性被降至较低，从而有益于快速、高通量的处理。与传统的多步骤 RNA 分离方案不同，无需加热、清洗或离心。该试剂盒大大简化了耗时费力的30–60流程，将其缩短至7分钟。

经过验证的卓越性能
SYBR Green Fast Advanced Cells-to-CT 试剂盒的所有组分均经过优化，具有一致性且可靠的性能。这样可以消除参与不同样品制备、RT 和实时荧光定量 PCR 试剂盒工作过程中的猜测，使您在现有的较快样品至结果工作流程中获得所需的灵敏度。

仅供科研使用。不可用于诊断程序。

规格

脱氧核糖核酸酶220 μL DNase I

保真度（相对于 Taq）2X

产品规格管装

高通量能力兼容高通量应用

反应次数400 次反应

产品线Cells-to-CT

产品类型Fast Advanced Cells-to-Ct 试剂盒

纯化目标裂解物

数量400 reactions

试剂类型细胞裂解

样品类型哺乳动物细胞

运输条件干冰

原始材料10-100,000 Cells

足够用于400 次反应

最大浓度2X

检测方法SYBR

适用于（应用）Gene Expression

高 GC PCR 扩增效果高

PCR 方法两步法 RT-qPCR

反应速度快速

Unit SizeEach

内容与储存

• 1 mL 20X Fast Advanced RT 酶混合物
• 20 mL 2X Fast Advanced RT 缓冲液
• 20 mL 裂解液
• 1 mL 终止液
• 220 µL DNase I
• 20 mL PowerUp SYBR Green 预混液

在 -5 至 -30°C 下储存。

常见问题解答 (FAQ)

我的特定细胞系能用Cells-to-CT系统吗？

Cells-to-CT系统应该能适用于所有细胞系。但是，由于细胞大小和成分的差异，每个裂解反应的最大细胞数在不同细胞系中会有轻微差异。您可使用TaqMan对照试剂盒检测抑制和最小样品起始量。

Will the Cells-to-CT system work with my special cell line?

There is no reason why the Cells-to-CT system shouldn’t work with any cell line. However, due to differences in cell size and composition, the maximum number of cells per lysis reaction may be slightly different for different cell lines. We recommend testing for inhibition and optimal cell input by using the TaqMan Cells-to-CT and SYBR Green Cells-to-CT Control kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Do the 2-step Cells-to-CT kits contain RNase inhibitor?

Yes, the Stop Solution provided in the 2-step Cells-to-CT kits contains RNase inhibitor.

Can I use the TaqMan Gene Expression Cells-to-CT kit for multiplexing?

The TaqMan Gene Expression Cells-to-CT kit has been validated for duplexing. If you want to set up a multiplex real-time PCR reaction with 3 assays, we recommend using the TaqMan Fast Advanced Cells-to-CT kit (https://www.thermofisher.com/order/catalog/product/A35374).

I am using a Cells-to-CT kit and I see signal from the genomic DNA in my real-time PCR results. How do I get rid of the genomic DNA contamination?

To prevent signal from genomic DNA in the Cells-to-CT real-time PCR reaction, we recommend using a TaqMan assay or primer set that spans an exon-exon boundary, and adding DNase I to degrade genomic DNA during the lysis reaction. For optimal DNase activity in the lysis reaction, we recommend the following:
1. Ensure all media is removed from the cells.
2. Wash each well or cell pellet with an equal volume of room temperature 1X PBS.
3. Ensure the lysis reaction happens at room temperature. The lysis reaction may not reach room temperature if the plate is on ice prior to adding Lysis Solution, or cold Lysis Solution is added.
4. Warm the Lysis Solution to room temperature before adding to the cells.
5. Perform the lysis reaction at 25 degrees C for up to 8 minutes.

View all SYBR™ Green Fast Advanced Cells-to-CT™ 试剂盒 FAQs