Cells-to-CT™ Bulk Fast Advanced RT Reagents
Cells-to-CT™ Bulk Fast Advanced RT Reagents
Invitrogen™

Cells-to-CT™ Bulk Fast Advanced RT Reagents

Green features
Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) inRead more
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Catalog NumberQuantity
A391102500 reactions
Catalog number A39110
Price (CNY)
83,884.00
Each
Add to cart
Quantity:
2500 reactions
Price (CNY)
83,884.00
Each
Add to cart
Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT kit (20X RT enzyme mix and 20X RT buffer). These reagents are sufficient for 2500 RT reactions. When combined with the Cells-to- CT Bulk Lysis Reagents, these reagents can be easily incorporated into automated, high-throughput applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)20X
Green FeaturesFewer resources used and less waste, Less hazardous
High-throughput CompatibilityHigh-throughput Compatible
No. of Reactions2500 Reactions
Product LineCells-to-CT
Product TypeBulk Fast Advanced Rt Reagent
Purification TargetLysate
Quantity2500 reactions
Sample TypeCell
Shipping ConditionDry Ice
Sufficient For2500 Reactions
Volume68 mL
Concentration20X
Detection MethodPrimer-probe
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
Reaction SpeedFast
Unit SizeEach
Contents & Storage
• 6.8 mL 20X Fast Advanced RT Enzyme Mix
• 68 mL 2X Fast Advanced RT Buffer

Store at -5° to -30°C.

Frequently asked questions (FAQs)

Will the Cells-to-CT system work with my special cell line?

There is no reason why the Cells-to-CT system shouldn’t work with any cell line. However, due to differences in cell size and composition, the maximum number of cells per lysis reaction may be slightly different for different cell lines. We recommend testing for inhibition and optimal cell input by using the TaqMan Cells-to-CT and SYBR Green Cells-to-CT Control kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the size of the cDNA produced by the reverse transcriptase in the Cells-to-CT Bulk Fast Advanced RT Reagents?

The reverse transcriptase in this kit can make cDNA up to 7 Kb. However, this was not specifically tested on Cells-to-Ct lysates.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

Do the 2-step Cells-to-CT kits contain RNase inhibitor?

Yes, the Stop Solution provided in the 2-step Cells-to-CT kits contains RNase inhibitor.

Can I use the TaqMan Gene Expression Cells-to-CT kit for multiplexing?

The TaqMan Gene Expression Cells-to-CT kit has been validated for duplexing. If you want to set up a multiplex real-time PCR reaction with 3 assays, we recommend using the TaqMan Fast Advanced Cells-to-CT kit (https://www.thermofisher.com/order/catalog/product/A35374).

I am using a Cells-to-CT kit and I see signal from the genomic DNA in my real-time PCR results. How do I get rid of the genomic DNA contamination?

To prevent signal from genomic DNA in the Cells-to-CT real-time PCR reaction, we recommend using a TaqMan assay or primer set that spans an exon-exon boundary, and adding DNase I to degrade genomic DNA during the lysis reaction. For optimal DNase activity in the lysis reaction, we recommend the following:
1. Ensure all media is removed from the cells.
2. Wash each well or cell pellet with an equal volume of room temperature 1X PBS.
3. Ensure the lysis reaction happens at room temperature. The lysis reaction may not reach room temperature if the plate is on ice prior to adding Lysis Solution, or cold Lysis Solution is added.
4. Warm the Lysis Solution to room temperature before adding to the cells.
5. Perform the lysis reaction at 25 degrees C for up to 8 minutes.

View all Cells-to-CT™ Bulk Fast Advanced RT Reagents FAQs