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Invitrogen
CD42a Monoclonal Antibody (GR-P), FITC, eBioscience™
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Product Details
11-0428-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
GR-P
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome.
Applications Reported: This GR-P antibody has been reported for use in flow cytometric analysis.
Applications Tested: This GR-P antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Single-chain membrane glycoprotein that forms a non-covalent complex with GPIb. (MW 23 kDa) Reactivity with resting and activated platelets, weakly on monocytes, megakaryocytes and attachment site for the platelet plasma membrane to the submembrane cytoskeleton. GPIb/IX complex, functions as the receptor for ristocetin-induced binding of von Willebrand factor and as the von Willebrand factor-depend adhesion receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD42a; Glycoprotein 9; GP-IX; Platelet glycoprotein IX; platelet glycoprotein IX precursor; Platelet GPIX; unnamed protein product
Gene Aliases: CD42a; GP9; GPIX
UniProt ID: (Human) Q14445
Entrez Gene ID: (Human) 2815
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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