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HLA-BC Monoclonal Antibody (B1.23.2), PE, eBioscience™
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HLA-BC Antibody (12-5935-42) in Flow
Cell reports. Medicine 2023 -
Reduced T cell activity against HLA-edited rejTs in vitro (A) Schematic representation of donor DNAs and guide RNA (gRNA) for CRISPR-Cas9 "scarless" first- and second-step editing. Black bar, stop codon of B2M ; red arrow, gRNA target site; black arrows, primers for genotyping. (B) Biallelic iPSC clone with bright EGFP expression identified by fluorescent imaging. Scale bar, 100 mum. (C) Marker integration ... View More
产品信息
12-5935-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
B1.23.2
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This B1.23.2 monoclonal antibody recognizes the human Human Leukocyte Antigens (HLA) B and C. Along with HLA-A, these proteins correspond to MHC Class I in humans. HLA-B and HLA-C are expressed on nearly all cell types and heterodimerize with beta-2 microglobulin at the cell surface. These molecular complexes play a critical role in the presentation of endoplasmic reticulum-derived peptides to immune cells.
Applications Reported: This B1.23.2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B1.23.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: allele B-3515; B*3509; cell surface glycoprotein; integral membrane protein; glycosylation aa 86, alpha domain 1 aa 1-24, alpha domain 2 aa 25-114, alpha domain 3 aa 207-298; glycosylation aa 86, alpha domain 1 aa 25-114, alpha domain 2 aa 115-206, alpha domain 3 aa 207-298; heavy chain; hla antigen; HLA B-1519; HLA class I antigen HLA-B; HLA class I histocompatibility antigen, B alpha chain; HLA protein allele B37; HLA protein allele B51; HLA protein allele B57; HLA protein allele B7; HLA-A; HLA-B; HLA-B*4002; HLA-B-07ZEL allele; HLA-B-1501; HLA-B-1516; HLA-B-1520 allele; HLA-B-15AOH allele; HLA-B-15ZEL allele; HLA-B-27KSH allele; HLA-B-67LAV allele; This submission reports an HLA-B-6701 variant (HLA-B-67LAV) that differs from the published B-6701 sequence, GenBank Accession Number L17005; HLA-B18; HLA-B27 peptide; HLA-B39; HLA-B41 variant; HLA-B61; HLA-Bw-39-DBU allele; Human leukocyte antigen B; leukocyte antigen class I-B; lymphocyte antigen; MHC class 1 antigen; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC class I molecule; MHC class I-like alpha chain membrane-bound type; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B44; MHC HLA-B7 heavy chain precursor; MHC HLA-Bw46; similar to Homo sapiens MHC HLA-B39 mRNA with GenBank Accession Number L42024.1; subtype B*3508 differs at residue 156 (Leu->Arg) with subtype B*3501; unnamed protein product
基因别名: AS; B-4901; HLA-B; HLAB
UniProt ID: (Human) P01889
Entrez Gene ID: (Human) 3106
positive regulation of T cell mediated cytotoxicity
adaptive immune response
immune system process
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of endogenous peptide antigen via MHC class Ib
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent
regulation of T cell anergy
defense response
immune response
detection of bacterium
antigen processing and presentation
regulation of interleukin-12 production
regulation of interleukin-6 production
protection from natural killer cell mediated cytotoxicity
innate immune response
regulation of dendritic cell differentiation
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