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Invitrogen
Nur77 Monoclonal Antibody (12.14), PE, eBioscience™
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图: 1 / 7
Nur77 Antibody (12-5965-82) in Flow
Mouse thymocytes were unstimulated (left) or stimulated for 3 hours with the Cell Stimulation Cocktail (Product # 00-4970-03) (right) then intracellularly stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and 0.5 µg of Anti-Mouse Nur77 PE using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.
产品信息
12-5965-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
12.14
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.
Applications Reported: This 12.14 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 12.14 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: hormone receptor; MGC9485; Nuclear hormone receptor NUR/77; Nuclear protein N10; Nuclear receptor subfamily 4 group A member 1; Orphan nuclear receptor HMR; Orphan receptor tr3
基因别名: Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; Nr4a1; Nur77; TIS1; TR3
UniProt ID: (Mouse) P12813
Entrez Gene ID: (Mouse) 15370
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
C4 zinc finger nuclear receptor
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
apoptotic process
response to inorganic substance
endothelial cell chemotaxis
skeletal muscle cell differentiation
cellular response to vascular endothelial growth factor stimulus
positive regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
steroid hormone mediated signaling pathway
cellular response to fibroblast growth factor stimulus
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to organic substance
