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Invitrogen
Mouse IgA Monoclonal Antibody (11-44-2), PE, eBioscience™
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Mouse IgA Antibody (12-5994-81) in Flow
Nature communications 2021 -
Fig. 1 RIG-I is recruited to DNA DSB sites and suppresses non-homologous end-joining. a A549 cells were treated with irradiation (IR, 10 Gy, 2 h). RIG-I protein levels in the cytosolic (C) and nuclear (N) fractions were detected by Western blot. b A549 cells were treated with IR (10 Gy) for the indicated times. RIG-I protein levels in the soluble and chromatin fractions were examined by Western blot. c ER-A... View More
产品信息
12-5994-81
产品规格
种属反应
Mouse
已发表种属
Bacteria,
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
11-44-2
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The 11-44-2 monoclonal antibody reacts with the alpha heavy chain of mouse IgA. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgM. IgA is expressed as a membrane-bound form as well as a soluble dimer. Fluorochrome conjugated 11-44-2 is used as a detection secondary for mouse IgA.
Applications Reported: 11-44-2 has been reported for use in flow cytometric analysis to detect the surface IgA on mouse B cells.
Applications Tested: This 11-44-2 antibody has been tested by flow cytometric analysis to detect a mouse IgA primary antibody and can be used at less than or equal to 0.2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse IgA is an immunoglobulin A antibody that is produced by B cells in response to an antigen. It is a crucial component of the mucosal immune system, which protects the body from pathogens that enter through mucosal surfaces such as the respiratory, gastrointestinal, and urogenital tracts. Mouse IgA is composed of two heavy chains and two light chains, and it is the most abundant immunoglobulin in mucosal secretions. It plays a vital role in neutralizing pathogens and preventing their attachment to mucosal surfaces. Mouse IgA is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
