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          • Primary Antibodies ›
          • BCL-2 Antibodies

          Invitrogen

          Bcl-2 Monoclonal Antibody (10C4), PE, eBioscience™

          1 Published Figure
          1 Reference
          View all (104) BCL-2 antibodies

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          Cite Bcl-2 Monoclonal Antibody (10C4), PE, eBioscience™

          Additional Information:

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          • Antibody Testing Data (1)
          • Published Figures (1)
          Bcl-2 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Bcl-2 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Bcl-2 Antibody (12-6992-42) in Flow

          Mouse thymocytes were surface stained with CD4 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-0042-82) and CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0081-82) followed by intracellular staining with Anti-Mouse/Rat Bcl-2 PE using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. CD4+ single positive (purple histogram), CD8+ single positive (orange histogram), and CD4+CD8+ double positive thymocytes (green histogram) were used for analysis. Mouse IgG1 kappa Isotype Control, PE (Produ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Bcl-2 Antibody in Flow Cytometry (Flow)
          Bcl-2 Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          12-6992-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.25 µg)/test
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          10C4

          Conjugate

          PE PE PE
          • Unconjugated
          • eFluor 450
          • eFluor 660
          • FITC
          • PE-Cyanine7
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2815053

          Product Specific Information

          Description: The 10C4 monoclonal antibody reacts with mouse and rat Bcl-2, also known as B cell lymphoma 2. Bcl-2 is the founding member of the Bcl-2 family of evolutionarily-conserved proteins that regulate cell death/survival. Members of the Bcl-2 family include the pro-apoptotic proteins BAD, Bax, and Bim, and the anti-apoptotic proteins Bcl-2, Bcl-xL, and Mcl-1. Bcl-2 inhibits apoptosis by preventing the release of cytochrome c from the mitochondria. Mutation of Bcl-2 or overexpression of Bcl-2 due to chromosomal translocation has been identified as a contributing factor in numerous cancers. In developing thymocytes, Bcl-2 is expressed at high levels in CD4-CD8- double negative and in CD4+ and CD8+ single positive thymocytes; Bcl-2 expression is greatly diminished in CD4+CD8+ double positive thymocytes.

          Applications Reported: This 10C4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This 10C4 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

          Target Information

          BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; bcl-2; Bcl-2alpha; Bcl2-like protein; OTTHUMP00000163680; unnamed protein product

          View more View less

          Gene Aliases: Bcl-2; C430015F12Rik; D630044D05Rik; D830018M01Rik

          View more View less

          UniProt ID: (Mouse) P10417, (Rat) P49950

          View more View less

          Entrez Gene ID: (Mouse) 12043, (Rat) 24224

          View more View less

          Function(s)
          protease binding protein binding channel activity channel inhibitor activity protein phosphatase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding macromolecular complex binding protein heterodimerization activity BH domain binding BH3 domain binding protein phosphatase 2A binding binding, bridging DNA-binding transcription factor binding
          Process(es)
          protein polyubiquitination cell morphogenesis ossification ovarian follicle development metanephros development ureteric bud development branching involved in ureteric bud morphogenesis behavioral fear response leukocyte homeostasis B cell homeostasis kidney development release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphocyte homeostasis lymphoid progenitor cell differentiation B cell lineage commitment T cell lineage commitment immune system development response to ischemia renal system process protein dephosphorylation melanin metabolic process regulation of nitrogen utilization cellular calcium ion homeostasis apoptotic process cellular response to DNA damage stimulus response to oxidative stress actin filament organization axonogenesis cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress apoptotic mitochondrial changes response to xenobiotic stimulus response to toxic substance post-embryonic development organ morphogenesis response to iron ion response to UV-B response to gamma radiation regulation of gene expression regulation of autophagy negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process negative regulation of cardiac muscle cell apoptotic process mesenchymal cell development positive regulation of neuron maturation positive regulation of smooth muscle cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cochlear nucleus development gland morphogenesis hemopoiesis B cell differentiation T cell differentiation negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development regulation of protein localization negative regulation of myeloid cell apoptotic process T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth regulation of growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to hydrogen peroxide regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process regulation of programmed cell death positive regulation of catalytic activity CD8-positive, alpha-beta T cell lineage commitment pigmentation regulation of neuron apoptotic process negative regulation of neuron apoptotic process ear development regulation of viral genome replication response to ethanol positive regulation of melanocyte differentiation negative regulation of mitotic cell cycle negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly developmental pigmentation regulation of developmental pigmentation positive regulation of developmental pigmentation spleen development thymus development response to steroid hormone digestive tract morphogenesis developmental growth oocyte development positive regulation of skeletal muscle fiber development pigment granule organization homeostasis of number of cells within a tissue regulation of catalytic activity B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus regulation of cell cycle regulation of mitochondrial membrane potential regulation of calcium ion transport transmembrane transport intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hypoxia reactive oxygen species metabolic process extrinsic apoptotic signaling pathway in absence of ligand cell-cell adhesion negative regulation of G1/S transition of mitotic cell cycle negative regulation of anoikis negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway response to hypoxia response to nutrient response to aluminum ion cerebral cortex development response to caffeine response to insulin response to vitamin E response to L-ascorbic acid response to monosaccharide glial cell apoptotic process response to erythropoietin swimming behavior response to peptide hormone response to estrogen response to copper ion oligodendrocyte differentiation response to corticosterone response to cAMP response to folic acid response to electrical stimulus cellular response to hydrogen peroxide cellular response to glucose stimulus response to anesthetic response to phenylpropanoid liver regeneration cellular response to fluoride response to amyloid-beta cellular response to L-glutamate response to insulin-like growth factor stimulus
          It has to be done as per old AB suggested Products section.

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