Search

  • Contact Us
  • Quick Order

Invitrogen

Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), PE, eBioscience™

4 Published Figures
12 References
View all (56) mTOR antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

12-9718-42

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test
View 11 publications 11 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MRRBY

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572724

Product Specific Information

Description: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. mTOR is a serine/threonine protein kinase that functions as an ATP and amino acid sensor as well as to balance nutrient availability with cell growth, proliferation, motility, survival, protein synthesis, and transcription. Activated mTOR increases production of enzymes necessary for glycolysis and controls the uptake of glucose and other nutrients. Increased glucose uptake and metabolism helps fulfill the energy needs for mTOR-driven cell growth and proliferation. When sufficient nutrients are available, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor, 4E-BP1. mTOR is phosphorylated at S2448 via the PI3 kinase/Akt signaling pathway and is autophosphorylated at Ser2481. Due to its critical role in regulation of cell growth, survival, and metabolism, and because it is often abnormally regulated in tumors, mTOR is under investigation as a potential target for anti-cancer therapy.

Applications Reported:This MRRBY antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MRRBY antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mTOR; mTORC1; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR; Tyrosine-protein kinase mTOR

View more View less

Gene Aliases: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1

View more View less

UniProt ID: (Mouse) Q9JLN9

View more View less

Entrez Gene ID: (Mouse) 56717

View more View less

Function(s)
nucleotide binding RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding TFIIIC-class transcription factor binding protein serine/threonine kinase activity protein binding ATP binding drug binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor protein domain specific binding ribosome binding protein dimerization activity phosphoprotein binding non-receptor serine/threonine protein kinase protein modifying enzyme
Process(es)
double-strand break repair via homologous recombination positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of glycogen biosynthetic process regulation of carbohydrate metabolic process protein phosphorylation response to stress germ cell development negative regulation of autophagy positive regulation of lamellipodium assembly positive regulation of gene expression positive regulation of myotube differentiation cell growth negative regulation of macroautophagy phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cell projection organization positive regulation of actin filament polymerization ruffle organization regulation of myelination cellular response to nutrient levels TOR signaling regulation of fatty acid beta-oxidation regulation of response to food response to insulin regulation of actin cytoskeleton organization regulation of GTPase activity response to amino acid regulation of carbohydrate utilization regulation of osteoclast differentiation positive regulation of translation negative regulation of cell size regulation of protein kinase activity positive regulation of transcription from RNA polymerase III promoter protein autophosphorylation positive regulation of lipid biosynthetic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of stress fiber assembly negative regulation of NFAT protein import into nucleus positive regulation of protein kinase B signaling cellular response to hypoxia
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support