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SAP (SLAM-Associated Protein) Monoclonal Antibody (XLP-1D12), PE, eBioscience™
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FIGURE: 1 / 5
SAP (SLAM-Associated Protein) Antibody (12-9787-42) in Flow
Intracellular staining of normal human peripheal blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or Anti-Human SAP PE (right), using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.
Product Details
12-9787-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
XLP-1D12
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody XLP-1D12 reacts with human SLAM-associated protein (SAP), which is also known as SH2D1A. SAP is a 15-kDa SH2 domain-containing adaptor protein. Upon CD150/SLAM family receptor ligation, SAP recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events. SAP is expressed in thymocytes, T cells, NK cells, and NKT cells. Studies have demonstrated a role for SAP in promoting follicular T helper cell differentiation during germinal center formation. In addition, this protein is involved in NKT cell development, Th1/Th2 differentiation, development of innate T cells, and regulation of NK cell and CD8+ T cell cytotoxicity. Mutations of the gene encoding SAP results in X-linked lymphoproliferative (XLP) syndrome, which is characterized by impaired humoral immunity leading to increased susceptibility to Epstein-Barr virus infection and/or lymphoma. SAP has also been linked to autoimmune diseases such as systemic lupus erythematosus.
Applications Reported: This XLP-1D12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This XLP-1D12 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources tab online. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Duncan disease SH2-protein; Duncan's disease; FLJ18687; FLJ92177; HGNC:10820; RP5-1052M9.3; SAP; SH2 domain-containing protein 1A; SH2D1A; signaling lymphocyte activation molecule-associated protein; Signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP; unnamed protein product
Gene Aliases: DSHP; EBVS; IMD5; LYP; MTCP1; SAP; SAP/SH2D1A; SH2D1A; XLP; XLPD; XLPD1
UniProt ID: (Human) O60880
Entrez Gene ID: (Human) 4068
adaptive immune response
immune system process
humoral immune response
cellular defense response
cell-cell signaling
natural killer cell activation
natural killer cell mediated cytotoxicity
innate immune response
positive regulation of natural killer cell mediated cytotoxicity
regulation of immune response
negative regulation of T cell receptor signaling pathway
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