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Invitrogen
E4BP4 (NFIL3) Monoclonal Antibody (MABA223), PE, eBioscience™
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FIGURE: 1 / 3
E4BP4 (NFIL3) Antibody (12-9812-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81) and Anti-Human CD28 Functional Grade Purified (Product # 16-0289-81) overnight (right) and then fixed and permeabilized with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). Cells were intracellularly stained with Anti-Human CD20 FITC (Product # 11-0209-42) and Anti-Human E4BP4 (NFIL3) PE. Viable cells, as determined by staining with Fixable Viability Dye eFluor® 780 (Prod... View More
Product Details
12-9812-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MABA223
Immunogen
21H2-hNFIL3 (aa 163-462)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This MABA223 monoclonal antibody recognizes human E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3. E4BP4 is involved in the transcriptional control of the mammalian circadian clock, motor neuron growth and survival, regulation of von Willbrand factor expression, and osteoblast function. In addition, it is required for the development of NK and NKT cells as well as CD8a+ dendritic cells. Although relatively high levels of mRNA are reported in resting NK and NKT cells, studies at eBioscience have observed expression of E4BP4 protein in lymphocytes only after activation.
Applications Reported: This MABA223 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MABA223 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NF IL3A; nuclear factor interleukin 3 regulated protein; Nuclear factor interleukin-3-regulated protein; OTTHUMP00000021633; Transcriptional activator NF-IL3A
Gene Aliases: E4BP4; IL3BP1; NF-IL3A; NFIL3; NFIL3A
UniProt ID: (Human) Q16649
Entrez Gene ID: (Human) 4783
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
basic leucine zipper transcription factor
DNA-binding transcription factor
gene-specific transcriptional regulator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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