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Invitrogen
E-cadherin Monoclonal Antibody (HECD-1)
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产品信息
13-1700
应用
建议稀释比
已发表文章
产品规格
种属反应
Guinea pig,
Human
已发表种属
Avian,
Bovine,
C. elegans,
Dog,
Fruit fly,
Human,
Insect,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
HECD-1
抗原
Human breast tumor cell line MCF-7.
偶联物
形式
Lyophilized
浓度
2.0 mg/mL
纯化类型
Ion-exchange chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell line with spleen cells of BALB/c mouse after immunization with human breast tumor cell line MCF-7.
Specificity: This antibody specifically reacts with human E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact.
Cross reactivity: This antibody reacts with guinea pig E-cadherin. This antibody does not react with bovine, dog, rat or chicken E-cadherin.
Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.
Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Ca2+ should be contained in all the buffers to stabilize cadherin antigen.
靶标信息
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin
基因别名: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO
UniProt ID: (Human) P12830
Entrez Gene ID: (Human) 999, (Guinea pig) 100726210
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
response to toxic substance
single organismal cell-cell adhesion
pituitary gland development
negative regulation of cell-cell adhesion
extracellular matrix disassembly
extracellular matrix organization
neuron projection development
adherens junction organization
response to drug
positive regulation of transcription factor import into nucleus
positive regulation of transcription, DNA-templated
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
establishment of protein localization to plasma membrane
