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Invitrogen
IL-17A Monoclonal Antibody (eBio17B7), Biotin, eBioscience™
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FIGURE: 1 / 97
IL-17A Antibody (13-7177-81) in IHC
Scientific reports 2019 -
Sections of the colon of adult male mice. ( A - C) Mallory's trichrome-stained sections (200x); collagen fibers (arrows). ( A ) Control group showing few collagen fibers, ( B ) DSS group showing numerous collagen fibers, ( C ) DSS+ AuNPs group showing some collagen fibers. ( D - F ) Alcian blue-stained sections (200x); goblet cells (g). ( D ) Control group with abundant goblet cells, ( E ) DSS group wi... View More
Product Details
13-7177-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
C. elegans,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio17B7
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio17B7 antibody reacts with mouse and rat IL-17A with no recognition of IL-17F. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.
IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.
Applications Reported: The eBio17B7 antibody has been reported useful for ELISA/ELISPOT detection.
Applications Tested: The biotinylated eBio17B7 antibody has been tested as the detection antibody in a sandwich ELISA (and ELISPOT assay) for analysis of mouse IL-17A in combination with the purified eBio17CK15A5 (14-7175) antibody for capture and recombinant mouse IL-17A (14-8171) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A
Gene Aliases: CTLA-8; Ctla8; IL-17; IL-17A; Il17; Il17a
UniProt ID: (Mouse) Q62386
Entrez Gene ID: (Mouse) 16171, (Rat) 301289
inflammatory response
positive regulation of necrotic cell death
positive regulation of interleukin-23 production
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to interleukin-1
cellular response to glucocorticoid stimulus
fibroblast activation
positive regulation of cytokine production involved in inflammatory response
response to amino acid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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