Invitrogen

Connexin 43 Monoclonal Antibody (CX-1B1)

Name: Connexin 43 Monoclonal Antibody (CX-1B1)
Brand: Invitrogen
SKU: 13-8300
Price: 5124.00 CNY
Availability: InStock

高级验证

80个已发表图片

180 篇参考文献

查看其他33个Connexin 43抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

Connexin 43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 88

Connexin 43 Antibody (13-8300) in IHC (P)

Immunohistochemistry analysis of Connexin 43 showing staining in the membrane of paraffin-embedded human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temp... View More

Connexin 43 Antibody in Immunohistochemistry (IHC)

Connexin 43 Antibody in Western Blot (WB)

Connexin 43 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Connexin 43 Antibody in Immunocytochemistry (ICC/IF)

Connexin 43 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

Connexin 43 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Connexin 43 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Connexin 43 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Connexin 43 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Immunogen image

Immunogen Image Connexin 43 Monoclonal Antibody (CX-1B1)

产品信息

13-8300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看67篇已发表文章 67篇已发表文章

免疫组化 (IHC)

查看36篇已发表文章 36篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

查看6篇已发表文章 6篇已发表文章

免疫组化（冰冻） (IHC (F))

查看10篇已发表文章 10篇已发表文章

免疫组织化学（悬浮细胞） (IHC (Free))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看43篇已发表文章 43篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-1 µg/mL

免疫沉淀 (IP)

查看4篇已发表文章 4篇已发表文章

免疫电镜技术 (IM)

Assay-dependent

其他PubMed (Misc)

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Bat, Cat, Dog, Hamster, Human, Mouse, Pig, Rabbit, Rat, Rodent, Sheep

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

CX-1B1

抗原

Synthetic peptide corresponding to a cytoplasmic sequence located near the C-terminus of rat Connexin 43.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

Ambient (domestic); Wet ice (international)

AB_2533038

产品详细信息

CX-1B1 is specific for one of the unphosphorylated forms of Connexin 43. This antibody was at one time thought to be specific for all unphosphorlyated forms of Connexin 43, but that can no longer be claimed since it was discovered that Connexin 43 can be phosphorylated at multiple sites. Recent studies have shown that phosphorylation occurs at a serine within the epitope recognized by this antibody. CX-1B1 recognizes Connexin 43 only when the serine at residue 368 is unphosphorylated. CX-1B1 will, however, recognize Connexin 43 if it is phosphorylated at sites besides Ser368. In some cell types, cross-reactivity with a ~70 kDa protein of unknown identity has been observed. This cross-reactivity is likely due to a shared epitope.

Reactivity has been confirmed by western blot analysis of extracts derived from dog heart, human heart, mouse brain, mouse heart, rat brain, and Clone 9 (C1-9) rat liver cells. Clone 9 is the recommended positive control for CX-1B1.

This antibody has also been used in immunofluorescence applications and immunolocalization of Connexin 43, however, for immunohistochemical applications polyclonal Rabbit anti-Connexin 43 (Cat. No. 71-0700) is recommended.

靶标信息

Connexin 43 (Cx43), encoded by the GJA1 gene, is a widely expressed gap junction protein that plays an important role in direct cell-to-cell communication. Six Cx43 proteins assemble to form a connexon, or hemichannel, which can pair with a connexon on a neighboring cell to create a gap junction channel. These channels allow ions, metabolites, and small signaling molecules to pass between adjacent cells, helping coordinate processes such as electrical conduction, tissue development, wound healing, and cellular homeostasis. Cx43 is especially abundant in tissues such as the heart, brain, bone, and skin, and changes in its expression, localization, or function have been associated with cardiovascular disease, neurological disorders, impaired tissue repair, and cancer. Because of its broad role in intercellular signaling and disease biology, Cx43 is an important target for studying gap junction function and developing potential therapeutic strategies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: alpha 1 connexin; connexin 43; connexin k1; connexin-43; Cx-43; CXA1; DFNB38; gap junction 43 kDa heart protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); GJA1; Npm1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product; VEGF

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基因别名: AVSD3; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxnk1; EKVP; EKVP3; Gja-1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Human) 2697, (Dog) 403418, (Mouse) 14609, (Rat) 24392

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