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Invitrogen

CD117 (c-Kit) Monoclonal Antibody (ACK2), eBioscience™

48 Published Figures
85 References
View all (156) c-Kit (CD117) antibodies

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Product Details

14-1172-82

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 4 publications 4 publications

Immunohistochemistry (IHC)

-
View 26 publications 26 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

-
View 14 publications 14 publications

Flow Cytometry (Flow)

0.125 µg/test
View 35 publications 35 publications

Immunoprecipitation (IP)

Assay-Dependent
-

Neutralization (Neu)

-
View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent
View 2 publications 2 publications

Inhibition Assays (Inhibition)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Chicken, Fish, Guinea pig, Human, Mouse, Rat

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

ACK2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467436

Product Specific Information

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported for use in functional assays. (Please use Functional Grade purified ACK2, Product # 16-1172, in functional assays.) (Fluorochrome-conjugated ACK2 is recommended for use in flow cytometric analysis).

Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: belly-spot; c-kit proto-oncogene protein; c-Kit protooncogene; c-kit receptor tyrosine kinase; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; protein kinase; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit; unnamed protein product; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Gene Aliases: Bs; C-Kit; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; MASTC; PBT; SCFR; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Human) P10721, (Mouse) P05532

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Entrez Gene ID: (Human) 3815, (Rat) 64030, (Mouse) 16590

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Function(s)
nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding kinase activity transferase activity growth factor binding cytokine binding SH2 domain binding protein homodimerization activity metal ion binding receptor signaling protein tyrosine kinase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor
Process(es)
ovarian follicle development hematopoietic progenitor cell differentiation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation immature B cell differentiation mast cell chemotaxis positive regulation of cytokine production involved in immune response positive regulation of dendritic cell cytokine production glycosphingolipid metabolic process chemotaxis inflammatory response signal transduction transmembrane receptor protein tyrosine kinase signaling pathway spermatogenesis spermatid development cell proliferation positive regulation of cell proliferation germ cell migration regulation of cell shape gonad development visual learning male gonad development response to radiation positive regulation of gene expression programmed cell death cell migration cytokine-mediated signaling pathway stem cell population maintenance lamellipodium assembly actin cytoskeleton organization hemopoiesis B cell differentiation T cell differentiation erythrocyte differentiation melanocyte differentiation positive regulation of cell migration positive regulation of pseudopodium assembly positive regulation of mast cell cytokine production somatic stem cell population maintenance embryonic hemopoiesis ectopic germ cell programmed cell death intracellular signal transduction hematopoietic stem cell migration megakaryocyte development Fc receptor signaling pathway Kit signaling pathway erythropoietin-mediated signaling pathway regulation of cell proliferation positive regulation of tyrosine phosphorylation of STAT protein negative regulation of programmed cell death mast cell degranulation positive regulation of MAPK cascade pigmentation tongue development leukocyte activation positive regulation of Notch signaling pathway positive regulation of JAK-STAT cascade response to cadmium ion protein autophosphorylation developmental pigmentation positive regulation of long-term neuronal synaptic plasticity digestive tract development stem cell differentiation epithelial cell proliferation regulation of developmental process detection of mechanical stimulus involved in sensory perception of sound positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of developmental process positive regulation of protein kinase B signaling cell chemotaxis mast cell differentiation mast cell proliferation positive regulation of mast cell proliferation melanocyte migration melanocyte adhesion positive regulation of pyloric antrum smooth muscle contraction regulation of bile acid metabolic process positive regulation of colon smooth muscle contraction positive regulation of small intestine smooth muscle contraction positive regulation of vascular smooth muscle cell differentiation negative regulation of reproductive process activation of MAPK activity dendritic cell cytokine production myeloid leukocyte differentiation protein phosphorylation peptidyl-tyrosine phosphorylation signal transduction by protein phosphorylation actin cytoskeleton reorganization mast cell cytokine production positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein positive regulation of MAP kinase activity regulation of developmental pigmentation somatic stem cell division cellular response to thyroid hormone stimulus germ cell development phosphorylation cell differentiation
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