Invitrogen

CD160 Monoclonal Antibody (eBioCNX46-3 (CNX46-3)), eBioscience™

Name: CD160 Monoclonal Antibody (eBioCNX46-3 (CNX46-3)), eBioscience\u2122
Brand: Invitrogen
SKU: 14-1601-81

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说明书

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技术支持

说明书

证书

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问题与答案

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说明书

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Additional Information:

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CD160 Antibody (14-1601-81) in Flow

C57BL/6 splenocytes were stained with 0.5 µg of Anti-Mouse CD160 Purified (Product # 14-1601-81) followed by F (ab')2 Anti-Rat IgG PE (Product # 12-4012) and subsequently with Anti-Mouse CD3e FITC (Product # 11-0031-82) and Anti-Mouse NK1-1 APC (Product # 17-5941-82). The histogram (right) demonstrates staining of Anti-Mouse CD160 Purified on NK1-1+CD3. cells (red histogram and NK1-1+CD3+ cells (green histogram), as gated in the dot plot (left).

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产品信息

14-1601-81

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

流式细胞分析 (Flow)

1 µg/test

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免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioCNX46-3 (CNX46-3)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

50 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_1210783

产品详细信息

Description: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRgammadelta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.

Applications Reported: This eBioCNX46-3 (CNX46-3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). (Please use Functional Grade purified eBioCNX46-3 (CNX46-3), Product # 16-1601, in functional assays).

Applications Tested: This eBioCNX46-3 (CNX46-3) antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. It is involved in cell proliferation, cell surface receptor signaling pathways, cellular defense response, and regulation of immune response.

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