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Invitrogen
CD170 (Siglec F) Monoclonal Antibody (1RNM44N), eBioscience™
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图: 1 / 13
CD170 (Siglec F) Antibody (14-1702-82) in IHC (F)
Immunohistochemistry of frozen mouse lung stained with 20 µg/mL of Anti-Mouse CD170 (Siglec F) Purified followed by 10 µg/mL of Anti-Rat IgG TRITC (red), 10 µg/mL of Anti-Mouse CD11c Alexa Fluor® 488 (green), colocalization appears yellow. Nuclei are stained with DAPI (blue).
产品信息
14-1702-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
1RNM44N
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells.
This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.
Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This 1RNM44N antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 20 µg/mL. This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
SIGLEC-F is a sialic acid-binding immunoglobulin-like lectin predominantly expressed on eosinophils in mice, where it serves as a functional paralog of human SIGLEC-8. It acts as an inhibitory receptor with ITIM motifs that recognize sialylated glycans and can induce eosinophil apoptosis, thereby helping regulate allergic inflammation and immune homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD170; sialic acid binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding lectin Siglec-F; Siglec-5; SIGLEC-8; Siglec-F
基因别名: mSiglec-F; Siglec5; Siglecf
UniProt ID: (Mouse) Q920G3
Entrez Gene ID: (Mouse) 233186
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