Invitrogen

14-3-3 epsilon Monoclonal Antibody (3G11G2)

Name: 14-3-3 epsilon Monoclonal Antibody (3G11G2)
Brand: Invitrogen
SKU: MA5-49207

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (14)

14-3-3 epsilon Antibody in Immunocytochemistry (ICC/IF)

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14-3-3 epsilon Antibody (MA5-49207) in ICC/IF

Immunocytochemistry analysis of 14-3-3 epsilon in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in 14-3-3 epsilon Monoclonal antibody (Product # MA5-49207) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilutio... View More

14-3-3 epsilon Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 epsilon Antibody in Western Blot (WB)

14-3-3 epsilon Antibody in Flow Cytometry (Flow)

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Immunogen Image 14-3-3 epsilon Monoclonal Antibody (3G11G2)

产品信息

MA5-49207

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

3G11G2

抗原

E.coli-derived human YWHAE recombinant protein (Position: M1-Q255).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074865

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human SH-SY5Y whole cell, human SW620 whole cell, human Raji whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human colorectal adenocarcinoma tissue, human liver cancer tissue, human tonsil tissue, human endometrial cancer tissue, human placenta tissue, human bladder epithelial carcinoma tissue, human laryngeal squamous cell carcinomas tissue, rat brain tissue. ICC/IF: Caco-2 cell. Flow: ANA-1 cell, NRK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 14-3-3 epsilon; 14-3-3 protein epsilon; 14-3-3E; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide

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基因别名: 14-3-3E; AU019196; HEL2; KCIP-1; MDCR; MDS; YWHAE

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UniProt ID: (Human) P62258, (Rat) P62260, (Mouse) P42655

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Entrez Gene ID: (Human) 7531, (Rat) 29753, (Mouse) 22627

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