Invitrogen

14-3-3 zeta Polyclonal Antibody

Name: 14-3-3 zeta Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-85403
Availability: InStock

查看其他23个14-3-3 zeta抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

14-3-3 zeta Antibody in Western Blot (WB)

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14-3-3 zeta Antibody (PA5-85403) in WB

Western Blot using 14-3-3 zeta Polyclonal Antibody (Product # PA5-85403). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. 12% SDS PAGE. 14-3-3 zeta Polyclonal Antibody (Product # PA5-85403) diluted at 1:1,000.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

PA5-85403

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2792545

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (85%), Pig (100%), Chicken (98%), Sheep (99%), Rhesus Monkey (100%), Bovine (100%).

Positive Control: NIH-3T3, JC.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。