Invitrogen

14-3-3 zeta Polyclonal Antibody

Name: 14-3-3 zeta Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-96742

查看其他21个14-3-3 zeta抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

14-3-3 zeta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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14-3-3 zeta Antibody (PA5-96742) in IHC (P)

Immunohistochemistry analysis of 14-3-3 zeta in paraffin-embedded rat kidney. Samples were incubated with 14-3-3 zeta Polyclonal antibody (Product # PA5-96742) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

14-3-3 zeta Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image 14-3-3 zeta Polyclonal Antibody

产品信息

PA5-96742

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 146-245 of human YWHAZ (NP_0033971)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.44 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2808544

产品详细信息

Positive Samples: HeLa, A-549, MCF7, Mouse kidney, Mouse brain, Mouse lung, Rat brain; Cellular Location: Cytoplasm, Melanosome

Immunogen sequence: QQAYQEAFEI SKKEMQPTHP IRLGLALNFS VFYYEILNSP EKACSLAKTA FDEAIAELDT LSEESYKDST LIMQLLRDNL TLWTSDTQGD EAEAGEGGEN

靶标信息

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 14-3-3 delta; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 1433Z; 143Z; epididymis luminal protein 4; epididymis secretory protein Li 3; epididymis secretory protein Li 93; KCIP-1; Mitochondrial import stimulation factor S1 subunit; phospholipase A2; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; SEZ-2; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide

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基因别名: 1110013I11Rik; 14-3-3-zeta; 14-3-3z; 14-3-3zeta; AI596267; AL022924; AU020854; HEL-S-3; HEL-S-93; HEL4; KCIP-1; Msfs1; YWHAD; YWHAZ

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UniProt ID: (Human) P63104, (Mouse) Q3TSF1, (Rat) P63102

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Entrez Gene ID: (Human) 7534, (Mouse) 22631, (Rat) 25578

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