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Invitrogen
CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow)")
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CD49b (Integrin alpha 2) Antibody (14-5971-85) in Flow
Staining of C57Bl/6 splenocytes with FITC anti-mouse NK-1.1 (Product # 11-5941-82) and 0.25 µg of Rat IgM, K Isotype Control Purified (Product # 14-4341-82) (left) or 0.25 µg of Anti-Mouse CD49b Purified (right) followed by Anti-Rat IgM PE (Product # 12-0990-82). Cells in the lymphocyte gate were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD49b (Integrin alpha 2) Antibody (14-5971-85) in Flow")
产品信息
14-5971-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Rat
/ IgM, kappa
分类
Monoclonal
类型
Antibody
克隆号
DX5
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5^+PK136^- and DX5^-PK136^+ cells.
Applications Reported: The DX5 antibody has been reported for use in flow cytometric analysis. This antibody does not block adhesion and function of NK cells, and is not useful for immunoprecipitation and immunohistochemical staining of mouse tissues.
Applications Tested: The DX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor
基因别名: CD49B; DX5; Itga2
UniProt ID: (Mouse) Q62469
Entrez Gene ID: (Mouse) 16398
positive regulation of leukocyte migration
substrate-dependent cell migration
hypotonic response
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
positive regulation of epithelial cell migration
positive regulation of alkaline phosphatase activity
positive regulation of smooth muscle cell migration
positive regulation of cell projection organization
cell-substrate adhesion
positive regulation of collagen biosynthetic process
positive regulation of collagen binding
cell adhesion mediated by integrin
collagen-activated signaling pathway
positive regulation of DNA binding
skin morphogenesis
establishment of protein localization
positive regulation of translation
positive regulation of cell adhesion
positive regulation of smooth muscle contraction
focal adhesion assembly
positive regulation of smooth muscle cell proliferation
positive regulation of inflammatory response
positive regulation of positive chemotaxis
detection of mechanical stimulus involved in sensory perception of pain
positive regulation of transmission of nerve impulse
positive regulation of phagocytosis, engulfment
