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Invitrogen
Actin (muscle) Monoclonal Antibody (HHF35), eBioscience™
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FIGURE: 1 / 3
Actin (muscle) Antibody (14-6496-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human pancreas using 10 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (left) or 10 µg/mL Anti-Actin (muscle) Purified (right) followed by biotinylated Anti-Mouse IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-6496-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Horse,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HHF35
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.
The HHF35 antibody recognizes human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine and non-human primate actin.
Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections and western blotting.
Applications Tested: This HHF35 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58) and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACTB; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin; alpha actin 1; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; B-actin; beta actin; beta-actin FE-3; cardiac actin; cardiac alpha actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; RP5-1068B5.2; skeletal alpha actin; skeletal muscle alpha-actin 1; smooth muscle alpha-actin; Smooth muscle gamma-actin; vascular smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMGA; VSCM
UniProt ID: (Human) P68133, (Bovine) P68138, (Pig) P68137, (Bovine) P62739, (Human) P62736, (Human) P68032, (Bovine) Q3ZC07, (Human) P63261, (Bovine) P63258, (Bovine) Q5E9B5, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269
Entrez Gene ID: (Horse) 100065454, (Human) 58, (Bovine) 281592, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Dog) 477587, (Bovine) 515610, (Horse) 100062175, (Human) 59, (Dog) 478250, (Human) 70, (Bovine) 533219, (Horse) 100057678, (Pig) 100152267, (Human) 71, (Dog) 475923, (Bovine) 404122, (Pig) 397653, (Horse) 100050139, (Bovine) 281595, (Pig) 100520667, (Human) 72, (Horse) 100057561, (Dog) 475792, (Guinea pig) 100716933, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Guinea pig) 100722917, (Rat) 29275, (Guinea pig) 100731953, (Mouse) 11464, (Mouse) 11465, (Guinea pig) 100731713, (Rat) 287876, (Mouse) 11468, (Guinea pig) 100716255, (Rat) 25365
structural constituent of cytoskeleton
protein binding
ATP binding
myosin binding
ADP binding
protein kinase binding
ATPase activity
ubiquitin protein ligase binding
identical protein binding
profilin binding
nucleotide binding
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
muscle contraction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
positive regulation of gene expression
cell growth
muscle filament sliding
skeletal muscle thin filament assembly
skeletal muscle fiber adaptation
response to steroid hormone
skeletal muscle fiber development
mesenchyme migration
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
apoptotic process
actin filament-based movement
actomyosin structure organization
actin-myosin filament sliding
response to drug
negative regulation of apoptotic process
response to ethanol
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
retina homeostasis
movement of cell or subcellular component
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
sarcomere organization
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
angiogenesis
morphogenesis of a polarized epithelium
positive regulation of cell migration
regulation of stress fiber assembly
regulation of focal adhesion assembly
positive regulation of wound healing
postsynaptic actin cytoskeleton organization
tight junction assembly
protein localization to bicellular tight junction
metabolic process
actin-mediated cell contraction
response to calcium ion
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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